Identification, cloning, and expression of the major capsid protein gene of human herpesvirus 6.
Affiliation
Department of Molecular Biology, Christie Hospital & Holt Radium Institute, Manchester, United Kingdom.Issue Date
1990-02
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Show full item recordAbstract
DNA sequence analysis of part of the human herpesvirus 6 (HHV-6) genome led to the identification of an open reading frame with amino acid sequence homology to the major capsid proteins (MCP) of other HHVs. DIAGON analysis showed that the closest homology was with human cytomegalovirus. Plasmids were constructed which were shown to express the HHV-6 MCP as either the entire open reading frame or as portions of it, and the recombinant-produced proteins were used to raise antisera. The antisera were shown by immunofluorescence to react with HHV-6-infected lymphoblastoid cells and in Western blots with a 135-kilodalton protein specific to HHV-6-infected cells. The recombinant protein expressed from the entire HHV-6 MCP gene was detected only weakly in Western blot assays with normal HHV-6-positive human sera as a probe.Citation
Identification, cloning, and expression of the major capsid protein gene of human herpesvirus 6. 1990, 64 (2):714-22 J. Virol.Journal
Journal of VirologyPubMed ID
2153237Type
ArticleLanguage
enISSN
0022-538XCollections
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