Introduction of the activated N-ras oncogene into human fibroblasts by retroviral vector induces morphological transformation and tumorigenicity.
Authors
Kinsella, Anne RFiszer-Maliszewska, Lucja
Mitchell, Erika L D
Guo, Ya-Ping
Fox, Margaret
Scott, David
Affiliation
Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.Issue Date
1990-10
Metadata
Show full item recordAbstract
The introduction of activated N-ras cDNA into normal diploid human skin fibroblast cell cultures using the retroviral vector pZIPneo results in a spectrum of morphologies ranging from near normal to, in rare instances, dense piled-up colonies of morphologically transformed cells. However, none of the clones isolated were transformed as assessed by growth on agar or tumorigenicity in nude mice. Introduction of both c-myc and N-ras oncogene cDNAs into normal skin fibroblasts failed to produce transformation as assessed by growth on agar and tumorigenicity in nude mice, although c-myc infection alone conferred immortality and the resultant doubly infected cell line was immortal. Using the same construct, activated N-ras cDNA was shown to transform immortalized human fibroblasts to tumorigenicity. However, immortalization per se was shown not to guarantee 'co-operation' with an activated N-ras gene to give malignant transformation. Although numerical and structural chromosome aberrations (clonal and non-clonal) were observed in some of the cell strains isolated after retroviral infection, these were not directly associated with viral infection, the presence of the oncogenes or with the morphologically transformed phenotype.Citation
Introduction of the activated N-ras oncogene into human fibroblasts by retroviral vector induces morphological transformation and tumorigenicity. 1990, 11 (10):1803-9 CarcinogenesisJournal
CarcinogenesisDOI
10.1093/carcin/11.10.1803PubMed ID
2208593Type
ArticleLanguage
enISSN
0143-3334ae974a485f413a2113503eed53cd6c53
10.1093/carcin/11.10.1803
Scopus Count
Collections
Related articles
- Malignant transformation of human fibroblasts by a transfected N-ras oncogene.
- Authors: Wilson DM, Yang DJ, Dillberger JE, Dietrich SE, Maher VM, McCormick JJ
- Issue date: 1990 Sep 1
- Multistep carcinogenesis of normal human fibroblasts. Human fibroblasts immortalized by repeated treatment with Co-60 gamma rays were transformed into tumorigenic cells with Ha-ras oncogenes.
- Authors: Namba M, Nishitani K, Fukushima F, Kimoto T
- Issue date: 1988 Sep-Oct
- Cooperation between the H-ras oncogene and a truncated derivative of the v-myb oncogene in transformation of hamster embryo fibroblasts.
- Authors: Merzak A, Dooghe Y, Pironin M, Perbal B, Vigier P
- Issue date: 1992 Oct
- Deregulated expression of human c-jun transforms primary rat embryo cells in cooperation with an activated c-Ha-ras gene and transforms rat-1a cells as a single gene.
- Authors: Schütte J, Minna JD, Birrer MJ
- Issue date: 1989 Apr
- Transformation and radiosensitivity of human diploid skin fibroblasts transfected with activated ras oncogene and SV40 T-antigen.
- Authors: Su LN, Little JB
- Issue date: 1992 Aug