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    Molecular cloning and characterization of a defective recombinant feline leukaemia virus associated with myeloid leukaemia.

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    Authors
    Tzavaras, T
    Stewart, M
    McDougall, A
    Fulton, R
    Testa, Nydia G
    Onions, D E
    Neil, J C
    Affiliation
    Beatson Institute for Cancer Research, Bearsden, Glasgow, U.K.
    Issue Date
    1990-02
    
    Metadata
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    Abstract
    The GM1 strain of feline leukaemia virus (FeLV) was isolated from a naturally occurring case of myeloid leukaemia and induces severe haematopoietic abnormalities, including myeloblastic leukaemia, on inoculation into cats. Molecular clones of FeLV-GM1 proviruses were obtained and studied by restriction enzyme mapping, blot hybridization and partial DNA sequence analysis. Two types of clone were isolated; the first was a replication-competent FeLV of subgroup A, resembling other low or minimally pathogenic FeLV-A isolates; the second was replication-defective with extensive deletions and mutations in gag and pol, although it has an intact env gene of subgroup B phenotype. Large segments of the defective proviruses, from the 5' leader sequence upstream of the gag gene to the 5' half of the env gene, show structural hallmarks of endogenous FeLV-related proviruses. Infectious FeLV-GM1 viruses recovered after transfection were tested for their leukaemogenic potential in newborn cats. Early polyclonal myeloproliferative changes were observed in cats inoculated with FeLV-A/GM1 alone, although these were more pronounced in animals receiving the full FeLV-AB/GM1 complex reconstituted by cotransfection of the defective virus FeLV-B with its FeLV-A helper. Analysis of viruses in the bone marrow showed that replication of the subgroup B component is delayed and restricted to a proportion of cats. Most of the infected cats developed persistent abnormalities of haematopoiesis and one progressed to disseminated myeloid leukaemia. The defective recombinant FeLV-B/GM1 appears to play an indirect but important role in myeloid leukaemogenesis.
    Citation
    Molecular cloning and characterization of a defective recombinant feline leukaemia virus associated with myeloid leukaemia. 1990, 71 ( Pt 2):343-54 J. Gen. Virol.
    Journal
    Journal of General Virology
    URI
    http://hdl.handle.net/10541/109807
    DOI
    10.1099/0022-1317-71-2-343
    PubMed ID
    2155287
    Type
    Article
    Language
    en
    ISSN
    0022-1317
    ae974a485f413a2113503eed53cd6c53
    10.1099/0022-1317-71-2-343
    Scopus Count
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    All Paterson Institute for Cancer Research

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