• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Modulation of extracellular matrix biosynthesis by bovine retinal pericytes in vitro: effects of the substratum and cell density.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Canfield, Ann E
    Allen, Terence D
    Grant, M E
    Schor, Seth L
    Schor, Ana M
    Affiliation
    Department of Medical Oncology, Paterson Institute, Christie Hospital and Holt Radium Institute, Manchester, UK.
    Issue Date
    1990-05
    
    Metadata
    Show full item record
    Abstract
    Bovine retinal pericytes plated on a two-dimensional substratum display a characteristic stellate morphology. In post-confluent cultures these cells aggregate spontaneously to form multicellular nodules. The same cells plated within a three-dimensional collagen matrix display an elongated sprouting morphology. Sprouting pericytes may be embedded within a gel either as individual cells or as multicellular aggregates. We have compared the nature of the matrix proteins synthesised by pericytes displaying these different phenotypes. Stellate pericytes cultured on plastic dishes synthesised predominantly type I collagen, some type III collagen and only traces of type IV collagen. The same collagen types were secreted when nodules had formed in postconfluent cultures on plastic, and by sprouting cells plated as single cells within the collagen gel. By contrast, sprouting pericytes plated as aggregates within the collagen gel secreted increased levels of type IV collagen and reduced amounts of type I collagen. Fibronectin was synthesized by pericytes under all experimental conditions examined; thrombospondin was produced in relatively large amounts by cells grown on plastic dishes, whereas only trace amounts could be detected in the medium when the cells were cultured within a collagen gel matrix. Transmission electron microscopy revealed that pericyte aggregates within a collagen gel contained cells in close apposition surrounded by a dense extracellular matrix. In contrast, cells in the centre of a nodule on plastic appeared to be separated from each other by loose extracellular material. These results suggest that the morphological and biosynthetic phenotypes of retinal pericytes are modulated by cell-matrix and/or cell-cell interactions.
    Citation
    Modulation of extracellular matrix biosynthesis by bovine retinal pericytes in vitro: effects of the substratum and cell density. 1990, 96 ( Pt 1):159-69 J. Cell. Sci.
    Journal
    Journal of Cell Science
    URI
    http://hdl.handle.net/10541/109797
    PubMed ID
    2373739
    Type
    Article
    Language
    en
    ISSN
    0021-9533
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Thrombospondin gene expression by endothelial cells in culture is modulated by cell proliferation, cell shape and the substratum.
    • Authors: Canfield AE, Boot-Handford RP, Schor AM
    • Issue date: 1990 May 15
    • Heterogeneity in collagen biosynthesis by sprouting retinal endothelial cells.
    • Authors: Canfield AE, Schor AM
    • Issue date: 1994 Apr
    • Pericyte differentiation.
    • Authors: Schor AM, Canfield AE, Sutton AB, Arciniegas E, Allen TD
    • Issue date: 1995 Apr
    • The biosynthesis of extracellular-matrix components by bovine retinal endothelial cells displaying distinctive morphological phenotypes.
    • Authors: Canfield AE, Schor AM, Schor SL, Grant ME
    • Issue date: 1986 Apr 15
    • Pericytes derived from the retinal microvasculature undergo calcification in vitro.
    • Authors: Schor AM, Allen TD, Canfield AE, Sloan P, Schor SL
    • Issue date: 1990 Nov
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.