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dc.contributor.authorLyon, Malcolm
dc.contributor.authorGallagher, John T
dc.date.accessioned2010-08-18T08:18:55Z
dc.date.available2010-08-18T08:18:55Z
dc.date.issued1990-02-15
dc.identifier.citationA general method for the detection and mapping of submicrogram quantities of glycosaminoglycan oligosaccharides on polyacrylamide gels by sequential staining with azure A and ammoniacal silver. 1990, 185 (1):63-70 Anal. Biochem.en
dc.identifier.issn0003-2697
dc.identifier.pmid1693050
dc.identifier.doi10.1016/0003-2697(90)90255-8
dc.identifier.urihttp://hdl.handle.net/10541/109793
dc.description.abstractA sensitive method has been developed for the visualization of nonradiolabeled glycosaminoglycan oligosaccharides resolved by polyacrylamide gel electrophoresis using fixation with azure A followed by staining with ammoniacal silver. This method, which can detect as little as 1-2 ng of a single oligosaccharide species, can be used to stain a few micrograms of a complex oligosaccharide mixture. The combination of gradient polyacrylamide gel electrophoresis and sequential azure A/silver staining can be applied to the analysis of all the complex glycosaminoglycans (i.e., heparin, heparan sulfate, chondroitin/dermatan sulfate, keratan sulfate) and hyaluronate, as well as to comparisons of specificities of the glycosaminoglycan-degrading enzymes. This procedure may be particularly valuable in situations where the availability of glycosaminoglycan is very limited and/or where radiolabeling is impractical or undesirable.
dc.language.isoenen
dc.subject.meshAzure Stains
dc.subject.meshChondroitin Sulfates
dc.subject.meshDermatan Sulfate
dc.subject.meshElectrophoresis, Polyacrylamide Gel
dc.subject.meshGlycosaminoglycans
dc.subject.meshHeparin
dc.subject.meshHeparitin Sulfate
dc.subject.meshKeratan Sulfate
dc.subject.meshOligosaccharides
dc.subject.meshParticle Size
dc.subject.meshPhenothiazines
dc.subject.meshPolysaccharide-Lyases
dc.subject.meshSilver
dc.subject.meshStaining and Labeling
dc.subject.meshSubstrate Specificity
dc.titleA general method for the detection and mapping of submicrogram quantities of glycosaminoglycan oligosaccharides on polyacrylamide gels by sequential staining with azure A and ammoniacal silver.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Medical Oncology, Christie Hospital, Manchester, United Kingdom.en
dc.identifier.journalAnalytical Biochemistryen
html.description.abstractA sensitive method has been developed for the visualization of nonradiolabeled glycosaminoglycan oligosaccharides resolved by polyacrylamide gel electrophoresis using fixation with azure A followed by staining with ammoniacal silver. This method, which can detect as little as 1-2 ng of a single oligosaccharide species, can be used to stain a few micrograms of a complex oligosaccharide mixture. The combination of gradient polyacrylamide gel electrophoresis and sequential azure A/silver staining can be applied to the analysis of all the complex glycosaminoglycans (i.e., heparin, heparan sulfate, chondroitin/dermatan sulfate, keratan sulfate) and hyaluronate, as well as to comparisons of specificities of the glycosaminoglycan-degrading enzymes. This procedure may be particularly valuable in situations where the availability of glycosaminoglycan is very limited and/or where radiolabeling is impractical or undesirable.


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