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    The synthesis of proteoglycans by human T lymphocytes.

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    Authors
    Steward, William P
    Christmas, Stephen E
    Lyon, Malcolm
    Gallagher, John T
    Affiliation
    CRC Dept. Medical Oncology, Christie Hospital, Manchester, U.K.
    Issue Date
    1990-05-22
    
    Metadata
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    Abstract
    We have examined the proteoglycans produced by highly-purified cultures of human T-lymphocytes. The proteoglycans were metabolically labelled with [35S]sulphate and analysed in cellular and medium fractions using DEAE-cellulose chromatography, gel filtration and specific enzymatic and chemical degradations. The results showed that the T cells synthesized a relatively homogeneous, proteinase-resistant chondroitin 4-sulphate proteoglycan that accumulated in the culture medium during a 48 h incubation period. The cellular fraction contained a significant amount of free chondroitin sulphate chains that were not secreted into the medium. These polysaccharides were formed by intracellular degradation of proteoglycan in a chloroquine-sensitive process, indicating a requirement for an acidic environment. In contrast to chondroitin sulphate derived from proteoglycan, chondroitin sulphates synthesized on the exogenous primer, beta-D-xyloside, were mainly secreted by the cells. beta-D-Xylosides caused an 8-fold stimulation in the synthesis of chondroitin sulphate, but decreased the synthesis of proteoglycan by about 50%. These proteoglycans contained shorter chondroitin sulphate chains than their normal counterparts. The results indicate that although proteoglycans are mainly secretory components in human T-cell cultures, a specific metabolic step leads to the intracellular accumulation of free glycosaminoglycans. Separate functions are likely to be associated with the intracellular and secretory pools of chondroitin sulphate.
    Citation
    The synthesis of proteoglycans by human T lymphocytes. 1990, 1052 (3):416-25 Biochim. Biophys. Acta
    Journal
    Biochimica et Biophysica Acta
    URI
    http://hdl.handle.net/10541/109769
    DOI
    10.1016/0167-4889(90)90151-3
    PubMed ID
    2354207
    Type
    Article
    Language
    en
    ISSN
    0006-3002
    ae974a485f413a2113503eed53cd6c53
    10.1016/0167-4889(90)90151-3
    Scopus Count
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    All Paterson Institute for Cancer Research

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