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dc.contributor.authorArciniegas, Een
dc.contributor.authorSutton, Andrew Ben
dc.contributor.authorAllen, Terence Den
dc.contributor.authorSchor, Ana Men
dc.date.accessioned2010-08-16T14:25:15Z
dc.date.available2010-08-16T14:25:15Z
dc.date.issued1992-10
dc.identifier.citationTransforming growth factor beta 1 promotes the differentiation of endothelial cells into smooth muscle-like cells in vitro. 1992, 103 ( Pt 2):521-9 J. Cell. Sci.en
dc.identifier.issn0021-9533
dc.identifier.pmid1478952
dc.identifier.urihttp://hdl.handle.net/10541/109655
dc.description.abstractAlpha-smooth muscle actin is considered a reliable marker for distinguishing between arterial smooth muscle and endothelial cells. Several authors have reported heterogeneity in the expression of this actin isoform in atherosclerotic lesions. Such heterogeneity appears to result from the presence of different smooth muscle cell phenotypes (contractile and synthetic) in these lesions. In the present study, we show that bovine aortic endothelial cells, which are characterised by the presence of Factor VIII-related antigen (FVIII) and by the absence of alpha-smooth muscle actin (alpha-SM actin) may be induced to express the latter when exposed to TGF-beta 1. FVIII was detected by immunofluorescence, alpha-SM actin was detected by immunofluorescence and immunoblotting. The number of cells expressing alpha-SM actin increased with time of incubation with TGF-beta 1, and this increase occurred concomitantly with a decrease in the expression of FVIII. Double immunofluorescence demonstrated the presence of cells that expressed both FVIII and alpha-SM actin after 5 days of incubation with TGF-beta 1. With longer incubation times (10-20 days) the loss of FVIII expression was complete and over 90% of the cells expressed alpha-SM actin. Ultrastructurally, cells in control cultures showed the typical features of endothelial cells. In the TGF-beta 1-treated cultures, cells which appeared indistinguishable from contractile and synthetic smooth muscle cells were observed. Withdrawal of TGF-beta 1 after 10 days incubation resulted in the re-appearance of polygonal cells which were FVIII-positive and alpha-SM actin-negative.(ABSTRACT TRUNCATED AT 250 WORDS)
dc.language.isoenen
dc.subject.meshActins
dc.subject.meshAnimals
dc.subject.meshAorta
dc.subject.meshCattle
dc.subject.meshCell Differentiation
dc.subject.meshCells, Cultured
dc.subject.meshEndothelium, Vascular
dc.subject.meshMicroscopy, Electron
dc.subject.meshMuscle, Smooth, Vascular
dc.subject.meshRetinal Vessels
dc.subject.meshTime Factors
dc.subject.meshTransforming Growth Factor beta
dc.subject.meshvon Willebrand Factor
dc.titleTransforming growth factor beta 1 promotes the differentiation of endothelial cells into smooth muscle-like cells in vitro.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Medical Oncology, Christie Hospital, Manchester, UK.en
dc.identifier.journalJournal of Cell Scienceen
html.description.abstractAlpha-smooth muscle actin is considered a reliable marker for distinguishing between arterial smooth muscle and endothelial cells. Several authors have reported heterogeneity in the expression of this actin isoform in atherosclerotic lesions. Such heterogeneity appears to result from the presence of different smooth muscle cell phenotypes (contractile and synthetic) in these lesions. In the present study, we show that bovine aortic endothelial cells, which are characterised by the presence of Factor VIII-related antigen (FVIII) and by the absence of alpha-smooth muscle actin (alpha-SM actin) may be induced to express the latter when exposed to TGF-beta 1. FVIII was detected by immunofluorescence, alpha-SM actin was detected by immunofluorescence and immunoblotting. The number of cells expressing alpha-SM actin increased with time of incubation with TGF-beta 1, and this increase occurred concomitantly with a decrease in the expression of FVIII. Double immunofluorescence demonstrated the presence of cells that expressed both FVIII and alpha-SM actin after 5 days of incubation with TGF-beta 1. With longer incubation times (10-20 days) the loss of FVIII expression was complete and over 90% of the cells expressed alpha-SM actin. Ultrastructurally, cells in control cultures showed the typical features of endothelial cells. In the TGF-beta 1-treated cultures, cells which appeared indistinguishable from contractile and synthetic smooth muscle cells were observed. Withdrawal of TGF-beta 1 after 10 days incubation resulted in the re-appearance of polygonal cells which were FVIII-positive and alpha-SM actin-negative.(ABSTRACT TRUNCATED AT 250 WORDS)


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