Show simple item record

dc.contributor.authorRooney, Claire M
dc.contributor.authorWhite, Gavin R M
dc.contributor.authorNazgiewicz, Alicja
dc.contributor.authorWoodcock, Simon A
dc.contributor.authorAnderson, Kurt I
dc.contributor.authorBallestrem, Christoph
dc.contributor.authorMalliri, Angeliki
dc.date.accessioned2010-08-10T15:31:34Z
dc.date.available2010-08-10T15:31:34Z
dc.date.issued2010-04
dc.identifier.citationThe Rac activator STEF (Tiam2) regulates cell migration by microtubule-mediated focal adhesion disassembly. 2010, 11 (4):292-8 EMBO Repen
dc.identifier.issn1469-3178
dc.identifier.pmid20224579
dc.identifier.doi10.1038/embor.2010.10
dc.identifier.urihttp://hdl.handle.net/10541/109433
dc.description.abstractFocal adhesion (FA) disassembly required for optimal cell migration is mediated by microtubules (MTs); targeting of FAs by MTs coincides with their disassembly. Regrowth of MTs, induced by removal of the MT destabilizer nocodazole, activates the Rho-like GTPase Rac, concomitant with FA disassembly. Here, we show that the Rac guanine nucleotide exchange factor (GEF) Sif and Tiam1-like exchange factor (STEF) is responsible for Rac activation during MT regrowth. Importantly, STEF is required for multiple targeting of FAs by MTs. As a result, FAs in STEF-knockdown cells have a reduced disassembly rate and are consequently enlarged. This leads to reduced speed of migration. Together, these findings suggest a new role for STEF in FA disassembly and cell migration through MT-mediated mechanisms.
dc.language.isoenen
dc.subjectTumour Cell Lineen
dc.subject.meshAnimals
dc.subject.meshCell Line, Tumor
dc.subject.meshCell Movement
dc.subject.meshFocal Adhesions
dc.subject.meshGuanine Nucleotide Exchange Factors
dc.subject.meshMice
dc.subject.meshMicroscopy, Fluorescence
dc.subject.meshMicrotubules
dc.subject.meshNocodazole
dc.subject.meshRNA Interference
dc.subject.meshrac GTP-Binding Proteins
dc.titleThe Rac activator STEF (Tiam2) regulates cell migration by microtubule-mediated focal adhesion disassembly.en
dc.typeArticleen
dc.contributor.departmentCell Signalling Group, Cancer Research UK Paterson Institute for Cancer Research, The University of Manchester, Manchester M20 4BX, UK.en
dc.identifier.journalEMBO Reportsen
refterms.dateFOA2020-05-01T14:47:46Z
html.description.abstractFocal adhesion (FA) disassembly required for optimal cell migration is mediated by microtubules (MTs); targeting of FAs by MTs coincides with their disassembly. Regrowth of MTs, induced by removal of the MT destabilizer nocodazole, activates the Rho-like GTPase Rac, concomitant with FA disassembly. Here, we show that the Rac guanine nucleotide exchange factor (GEF) Sif and Tiam1-like exchange factor (STEF) is responsible for Rac activation during MT regrowth. Importantly, STEF is required for multiple targeting of FAs by MTs. As a result, FAs in STEF-knockdown cells have a reduced disassembly rate and are consequently enlarged. This leads to reduced speed of migration. Together, these findings suggest a new role for STEF in FA disassembly and cell migration through MT-mediated mechanisms.


Files in this item

Thumbnail
Name:
embor.2010.10.pdf
Size:
228.1Kb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record