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    The Rac activator STEF (Tiam2) regulates cell migration by microtubule-mediated focal adhesion disassembly.

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    Authors
    Rooney, Claire M
    White, Gavin R M
    Nazgiewicz, Alicja
    Woodcock, Simon A
    Anderson, Kurt I
    Ballestrem, Christoph
    Malliri, Angeliki
    Affiliation
    Cell Signalling Group, Cancer Research UK Paterson Institute for Cancer Research, The University of Manchester, Manchester M20 4BX, UK.
    Issue Date
    2010-04
    
    Metadata
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    Abstract
    Focal adhesion (FA) disassembly required for optimal cell migration is mediated by microtubules (MTs); targeting of FAs by MTs coincides with their disassembly. Regrowth of MTs, induced by removal of the MT destabilizer nocodazole, activates the Rho-like GTPase Rac, concomitant with FA disassembly. Here, we show that the Rac guanine nucleotide exchange factor (GEF) Sif and Tiam1-like exchange factor (STEF) is responsible for Rac activation during MT regrowth. Importantly, STEF is required for multiple targeting of FAs by MTs. As a result, FAs in STEF-knockdown cells have a reduced disassembly rate and are consequently enlarged. This leads to reduced speed of migration. Together, these findings suggest a new role for STEF in FA disassembly and cell migration through MT-mediated mechanisms.
    Citation
    The Rac activator STEF (Tiam2) regulates cell migration by microtubule-mediated focal adhesion disassembly. 2010, 11 (4):292-8 EMBO Rep
    Journal
    EMBO Reports
    URI
    http://hdl.handle.net/10541/109433
    DOI
    10.1038/embor.2010.10
    PubMed ID
    20224579
    Type
    Article
    Language
    en
    ISSN
    1469-3178
    ae974a485f413a2113503eed53cd6c53
    10.1038/embor.2010.10
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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