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dc.contributor.authorBlankley, R T
dc.contributor.authorRobinson, N J
dc.contributor.authorAplin, J D
dc.contributor.authorCrocker, I P
dc.contributor.authorGaskell, S J
dc.contributor.authorWhetton, Anthony D
dc.contributor.authorBaker, P N
dc.contributor.authorMyers, J E
dc.date.accessioned2010-08-10T15:39:14Z
dc.date.available2010-08-10T15:39:14Z
dc.date.issued2010-03
dc.identifier.citationA gel-free quantitative proteomics analysis of factors released from hypoxic-conditioned placentae. 2010, 17 (3):247-57 Reprod Scien
dc.identifier.issn1933-7205
dc.identifier.pmid19907055
dc.identifier.doi10.1177/1933719109351320
dc.identifier.urihttp://hdl.handle.net/10541/109416
dc.description.abstractCharacterizing the protein factors released from placentae during pathogenesis remains a key objective toward understanding preeclampsia and related pregnancy disorders. Gel-free proteomics technologies applied to placental explant-conditioned media offers the potential of identifying these factors. Relative quantification mass spectrometry using isobaric tagging for relative and absolute quantification (iTRAQ) labeling was employed to compare the ''secretome'' between healthy term placental tissue cultured under both normoxic and hypoxic oxygen tensions. Of the 499 proteins identified, 45 were differentially expressed (P < .01 level), including interleukin 8 (IL-8) which was significantly upregulated under hypoxia. Global protein level changes are suggestive of decreased extracellular matrix remodeling under the same conditions. A significant enrichment of soluble liberated placental factors is achieved using this model system. Identifying these changes resulting from hypoxic conditioning is hypothesis generating and may provide new mechanistic insights into preeclampsia.
dc.language.isoenen
dc.subject.meshAnoxia
dc.subject.meshCulture Media, Conditioned
dc.subject.meshExtracellular Matrix
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshInterleukin-8
dc.subject.meshOxygen
dc.subject.meshPlacenta
dc.subject.meshPre-Eclampsia
dc.subject.meshPregnancy
dc.subject.meshProteins
dc.subject.meshProteomics
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
dc.subject.meshTissue Culture Techniques
dc.titleA gel-free quantitative proteomics analysis of factors released from hypoxic-conditioned placentae.en
dc.typeArticleen
dc.contributor.departmentMaternal and Fetal Health Research Group, St Mary's Hospital, Manchester, UK. richard.blankley-2@manchester.ac.uken
dc.identifier.journalReproductive Sciencesen
html.description.abstractCharacterizing the protein factors released from placentae during pathogenesis remains a key objective toward understanding preeclampsia and related pregnancy disorders. Gel-free proteomics technologies applied to placental explant-conditioned media offers the potential of identifying these factors. Relative quantification mass spectrometry using isobaric tagging for relative and absolute quantification (iTRAQ) labeling was employed to compare the ''secretome'' between healthy term placental tissue cultured under both normoxic and hypoxic oxygen tensions. Of the 499 proteins identified, 45 were differentially expressed (P < .01 level), including interleukin 8 (IL-8) which was significantly upregulated under hypoxia. Global protein level changes are suggestive of decreased extracellular matrix remodeling under the same conditions. A significant enrichment of soluble liberated placental factors is achieved using this model system. Identifying these changes resulting from hypoxic conditioning is hypothesis generating and may provide new mechanistic insights into preeclampsia.


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