Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid.
Spasić, Snezana D
Jones, David R
Spasić, Mihajlo B
AffiliationInstitute for Multidisciplinary Research, University of Belgrade, 11000 Belgrade, Serbia. firstname.lastname@example.org
MetadataShow full item record
AbstractA breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the prooxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.
CitationBioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. 2010, 15 (1):29-35 Redox Rep
- The role of chelating agents and amino acids in preventing free radical formation in bleaching systems.
- Authors: Hodes J, Sielaff P, Metz H, Kessler-Becker D, Gassenmeier T, Neubert RHH
- Issue date: 2018 Dec
- Iron-chelating agents never suppress Fenton reaction but participate in quenching spin-trapped radicals.
- Authors: Li L, Abe Y, Kanagawa K, Shoji T, Mashino T, Mochizuki M, Tanaka M, Miyata N
- Issue date: 2007 Sep 19
- The effects of chelating agents on radical generation in alkaline peroxide systems, and the relevance to substrate damage.
- Authors: Fowles EH, Gilbert BC, Giles MR, Whitwood AC
- Issue date: 2007 May
- The hydrolysis product of ICRF-187 promotes iron-catalysed hydroxyl radical production via the Fenton reaction.
- Authors: Thomas C, Vile GF, Winterbourn CC
- Issue date: 1993 May 25
- Reactions of copper(II)-N-polycarboxylate complexes with hydrogen peroxide in the presence of biological reductants: ESR evidence for the formation of hydroxyl radical.
- Authors: Ozawa T, Hanaki A, Onodera K, Kasai M
- Issue date: 1992 Mar