Quantitative and qualitative alterations of heparan sulfate in fibrogenic liver diseases and hepatocellular cancer.
Authors
Tátrai, PéterEgedi, Krisztina
Somorácz, Aron
Van Kuppevelt, Toin H
Ten Dam, Gerdy
Lyon, Malcolm
Deakin, Jon A
Kiss, András
Schaff, Zsuzsa
Kovalszky, Ilona
Affiliation
Second Department of Pathology, Semmelweis University, 93 Ulloi út H-1091 Budapest, Hungary. peter.tatrai@biomembrane.huIssue Date
2010-05
Metadata
Show full item recordAbstract
Heparan sulfate (HS), due to its ability to interact with a multitude of HS-binding factors, is involved in a variety of physiological and pathological processes. Remarkably diverse fine structure of HS, shaped by non-exhaustive enzymatic modifications, influences the interaction of HS with its partners. Here we characterized the HS profile of normal human and rat liver, as well as alterations of HS related to liver fibrogenesis and carcinogenesis, by using sulfation-specific antibodies. The HS immunopattern was compared with the immunolocalization of selected HS proteoglycans. HS samples from normal liver and hepatocellular carcinoma (HCC) were subjected to disaccharide analysis. Expression changes of nine HS-modifying enzymes in human fibrogenic diseases and HCC were measured by quantitative RT-PCR. Increased abundance and altered immunolocalization of HS was paralleled by elevated mRNA levels of HS-modifying enzymes in the diseased liver. The strong immunoreactivity of the normal liver for 3-O-sulfated epitope further increased with disease, along with upregulation of 3-OST-1. Modest 6-O-undersulfation of HCC HS is probably explained by Sulf overexpression. Our results may prompt further investigation of the role of highly 3-O-sulfated and partially 6-O-desulfated HS in pathological processes such as hepatitis virus entry and aberrant growth factor signaling in fibrogenic liver diseases and HCC.Citation
Quantitative and qualitative alterations of heparan sulfate in fibrogenic liver diseases and hepatocellular cancer. 2010, 58 (5):429-41 J. Histochem. Cytochem.Journal
The Journal of Histochemistry and CytochemistryDOI
10.1369/jhc.2010.955161PubMed ID
20124094Type
ArticleLanguage
enISSN
1551-5044ae974a485f413a2113503eed53cd6c53
10.1369/jhc.2010.955161
Scopus Count
Collections
Related articles
- Comparison of the expression of agrin, a basement membrane heparan sulfate proteoglycan, in cholangiocarcinoma and hepatocellular carcinoma.
- Authors: Batmunkh E, Tátrai P, Szabó E, Lódi C, Holczbauer A, Páska C, Kupcsulik P, Kiss A, Schaff Z, Kovalszky I
- Issue date: 2007 Oct
- [Selective deposition of agrin in the microvasculature of hepatocellular carcinoma: aspects in pathogenesis and differential diagnosis].
- Authors: Tátrai P
- Issue date: 2008 Dec
- Overexpression of heparan sulfate 6-O-sulfotransferases in human embryonic kidney 293 cells results in increased N-acetylglucosaminyl 6-O-sulfation.
- Authors: Do AT, Smeds E, Spillmann D, Kusche-Gullberg M
- Issue date: 2006 Mar 3
- Enhanced glypican-3 expression differentiates the majority of hepatocellular carcinomas from benign hepatic disorders.
- Authors: Zhu ZW, Friess H, Wang L, Abou-Shady M, Zimmermann A, Lander AD, Korc M, Kleeff J, Büchler MW
- Issue date: 2001 Apr
- Acute UV irradiation increases heparan sulfate proteoglycan levels in human skin.
- Authors: Jung JY, Oh JH, Kim YK, Shin MH, Lee D, Chung JH
- Issue date: 2012 Mar