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    A key role for Ctf4 in coupling the MCM2-7 helicase to DNA polymerase alpha within the eukaryotic replisome.

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    Authors
    Gambus, Agnieszka
    Van Deursen, Frederick
    Polychronopoulos, Dimitrios
    Foltman, Magdalena
    Jones, Richard C
    Edmondson, Ricky D
    Calzada, Arturo
    Labib, Karim
    Affiliation
    Cancer Research UK, Paterson Institute for Cancer Research, University of Manchester, Manchester, UK.
    Issue Date
    2009-10-07
    
    Metadata
    Show full item record
    Abstract
    The eukaryotic replisome is a crucial determinant of genome stability, but its structure is still poorly understood. We found previously that many regulatory proteins assemble around the MCM2-7 helicase at yeast replication forks to form the replisome progression complex (RPC), which might link MCM2-7 to other replisome components. Here, we show that the RPC associates with DNA polymerase alpha that primes each Okazaki fragment during lagging strand synthesis. Our data indicate that a complex of the GINS and Ctf4 components of the RPC is crucial to couple MCM2-7 to DNA polymerase alpha. Others have found recently that the Mrc1 subunit of RPCs binds DNA polymerase epsilon, which synthesises the leading strand at DNA replication forks. We show that cells lacking both Ctf4 and Mrc1 experience chronic activation of the DNA damage checkpoint during chromosome replication and do not complete the cell cycle. These findings indicate that coupling MCM2-7 to replicative polymerases is an important feature of the regulation of chromosome replication in eukaryotes, and highlight a key role for Ctf4 in this process.
    Citation
    A key role for Ctf4 in coupling the MCM2-7 helicase to DNA polymerase alpha within the eukaryotic replisome. 2009, 28 (19):2992-3004 EMBO J.
    Journal
    The EMBO Journal
    URI
    http://hdl.handle.net/10541/109323
    DOI
    10.1038/emboj.2009.226
    PubMed ID
    19661920
    Type
    Article
    Language
    en
    ISSN
    1460-2075
    ae974a485f413a2113503eed53cd6c53
    10.1038/emboj.2009.226
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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