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    Resonant Mie scattering (RMieS) correction of infrared spectra from highly scattering biological samples.

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    Authors
    Bassan, Paul
    Kohler, Achim
    Martens, Harald
    Lee, Joe
    Byrne, Hugh J
    Dumas, Paul
    Gazi, Ehsan
    Brown, Michael D
    Clarke, Noel W
    Gardner, Peter
    Affiliation
    School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, UK M1 7DN.
    Issue Date
    2010-02
    
    Metadata
    Show full item record
    Abstract
    Infrared spectra of single biological cells often exhibit the 'dispersion artefact' observed as a sharp decrease in intensity on the high wavenumber side of absorption bands, in particular the Amide I band at approximately 1655 cm(-1), causing a downward shift of the true peak position. The presence of this effect makes any biochemical interpretation of the spectra unreliable. Recent theory has shed light on the origins of the 'dispersion artefact' which has been attributed to resonant Mie scattering (RMieS). In this paper a preliminary algorithm for correcting RMieS is presented and evaluated using simulated data. Results show that the 'dispersion artefact' appears to be removed; however, the correction is not perfect. An iterative approach was subsequently implemented whereby the reference spectrum is improved after each iteration, resulting in a more accurate correction. Consequently the corrected spectra become increasingly more representative of the pure absorbance spectra. Using this correction method reliable peak positions can be obtained.
    Citation
    Resonant Mie scattering (RMieS) correction of infrared spectra from highly scattering biological samples. 2010, 135 (2):268-77 Analyst
    Journal
    The Analyst
    URI
    http://hdl.handle.net/10541/109311
    DOI
    10.1039/b921056c
    PubMed ID
    20098758
    Type
    Article
    Language
    en
    ISSN
    1364-5528
    ae974a485f413a2113503eed53cd6c53
    10.1039/b921056c
    Scopus Count
    Collections
    All Christie Publications
    All Paterson Institute for Cancer Research

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