Resonant Mie scattering (RMieS) correction of infrared spectra from highly scattering biological samples.
Authors
Bassan, PaulKohler, Achim
Martens, Harald
Lee, Joe
Byrne, Hugh J
Dumas, Paul
Gazi, Ehsan
Brown, Michael D
Clarke, Noel W
Gardner, Peter
Affiliation
School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, UK M1 7DN.Issue Date
2010-02
Metadata
Show full item recordAbstract
Infrared spectra of single biological cells often exhibit the 'dispersion artefact' observed as a sharp decrease in intensity on the high wavenumber side of absorption bands, in particular the Amide I band at approximately 1655 cm(-1), causing a downward shift of the true peak position. The presence of this effect makes any biochemical interpretation of the spectra unreliable. Recent theory has shed light on the origins of the 'dispersion artefact' which has been attributed to resonant Mie scattering (RMieS). In this paper a preliminary algorithm for correcting RMieS is presented and evaluated using simulated data. Results show that the 'dispersion artefact' appears to be removed; however, the correction is not perfect. An iterative approach was subsequently implemented whereby the reference spectrum is improved after each iteration, resulting in a more accurate correction. Consequently the corrected spectra become increasingly more representative of the pure absorbance spectra. Using this correction method reliable peak positions can be obtained.Citation
Resonant Mie scattering (RMieS) correction of infrared spectra from highly scattering biological samples. 2010, 135 (2):268-77 AnalystJournal
The AnalystDOI
10.1039/b921056cPubMed ID
20098758Type
ArticleLanguage
enISSN
1364-5528ae974a485f413a2113503eed53cd6c53
10.1039/b921056c
Scopus Count
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