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dc.contributor.authorKenessey, Istvánen
dc.contributor.authorSimon, Erikaen
dc.contributor.authorFutosi, Krisztinaen
dc.contributor.authorBereczky, Bíborkaen
dc.contributor.authorKiss, Andreaen
dc.contributor.authorErdödi, Ferencen
dc.contributor.authorGallagher, John Ten
dc.contributor.authorTímár, Józsefen
dc.contributor.authorTóvári, Józsefen
dc.date.accessioned2010-08-09T16:02:49Z
dc.date.available2010-08-09T16:02:49Z
dc.date.issued2009-12
dc.identifier.citationAntimigratory and antimetastatic effect of heparin-derived 4-18 unit oligosaccharides in a preclinical human melanoma metastasis model. 2009, 102 (6):1265-73 Thromb. Haemost.en
dc.identifier.issn0340-6245
dc.identifier.pmid19967160
dc.identifier.doi10.1160/TH09-01-0059
dc.identifier.urihttp://hdl.handle.net/10541/109309
dc.description.abstractHeparin and its derivatives have been shown to inhibit angiogenesis and metastasis formation. Accordingly, we investigated the effect of heparin fragments containing 4 to 22 monomers on human melanoma cell proliferation, migration and invasion in vitro as well as on the in vivo metastatic potential in a SCID mouse model. Only oligosaccharide dp18 had significant inhibitory effect on cell proliferation. In contrast, cell migration was inhibited by all oligosaccharides studied except dp8 and dp22. Anti-CD44v3 antibody stimulated cell migration and invasion, and this effect could be attenuated by oligosaccharides dp4 and dp18. These fragments also inhibited the catalytic activity of myosin light chain phosphatase as well. Moreover, oligosaccharides dp4 and dp18 reduced the number of lung colonies formed in SCID mice intravenously injected with human melanoma cells, while dp22 proved to be ineffective in this respect. These studies revealed that fragments of heparin have an antimigratory and antimetastatic potential. These fragments lack the haemostatic effect of heparin, suggesting that they are potential specific antimetastatic agents in anticancer therapy.
dc.language.isoenen
dc.subjectAnticancerous Agentsen
dc.subjectTumour Cell Lineen
dc.subjectLung Canceren
dc.subjectCancer Invasivenessen
dc.subjectCancer Transplantationen
dc.subject.meshAnimals
dc.subject.meshAntigens, CD44
dc.subject.meshAntineoplastic Agents
dc.subject.meshCell Line, Tumor
dc.subject.meshCell Movement
dc.subject.meshHeparin, Low-Molecular-Weight
dc.subject.meshHumans
dc.subject.meshLung Neoplasms
dc.subject.meshMelanoma, Experimental
dc.subject.meshMice
dc.subject.meshMice, SCID
dc.subject.meshNeoplasm Invasiveness
dc.subject.meshNeoplasm Transplantation
dc.subject.meshOligosaccharides
dc.subject.meshProtein Phosphatase 1
dc.subject.meshTransplantation, Heterologous
dc.titleAntimigratory and antimetastatic effect of heparin-derived 4-18 unit oligosaccharides in a preclinical human melanoma metastasis model.en
dc.typeArticleen
dc.contributor.departmentDepartment of Tumor Progression, National Institute of Oncology, Budapest, Hungary.en
dc.identifier.journalThrombosis and Haemostasisen
html.description.abstractHeparin and its derivatives have been shown to inhibit angiogenesis and metastasis formation. Accordingly, we investigated the effect of heparin fragments containing 4 to 22 monomers on human melanoma cell proliferation, migration and invasion in vitro as well as on the in vivo metastatic potential in a SCID mouse model. Only oligosaccharide dp18 had significant inhibitory effect on cell proliferation. In contrast, cell migration was inhibited by all oligosaccharides studied except dp8 and dp22. Anti-CD44v3 antibody stimulated cell migration and invasion, and this effect could be attenuated by oligosaccharides dp4 and dp18. These fragments also inhibited the catalytic activity of myosin light chain phosphatase as well. Moreover, oligosaccharides dp4 and dp18 reduced the number of lung colonies formed in SCID mice intravenously injected with human melanoma cells, while dp22 proved to be ineffective in this respect. These studies revealed that fragments of heparin have an antimigratory and antimetastatic potential. These fragments lack the haemostatic effect of heparin, suggesting that they are potential specific antimetastatic agents in anticancer therapy.


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