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    Regulation of breast cancer stem cell activity by signaling through the Notch4 receptor.

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    Authors
    Harrison, Hannah
    Farnie, Gillian
    Howell, Sacha J
    Rock, Rebecca E
    Stylianou, Spyros
    Brennan, Keith
    Bundred, Nigel J
    Clarke, Robert B
    Affiliation
    Breast Biology Group, School of Cancer, Enabling Sciences and Technology, Paterson Institute for Cancer Research, University of Manchester, Manchester Academic Health Sciences Centre, The Christie NHS Foundation Trust; Department of Medical Oncology, The Christie NHS Foundation Trust, Manchester M20 4BX, United Kingdom.
    Issue Date
    2010-01-15
    
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    Abstract
    Notch receptor signaling pathways play an important role not only in normal breast development but also in breast cancer development and progression. We assessed the role of Notch receptors in stem cell activity in breast cancer cell lines and nine primary human tumor samples. Stem cells were enriched by selection of anoikis-resistant cells or cells expressing the membrane phenotype ESA(+)/CD44(+)/CD24(low). Using these breast cancer stem cell populations, we compared the activation status of Notch receptors with the status in luminally differentiated cells, and we evaluated the consequences of pathway inhibition in vitro and in vivo. We found that Notch4 signaling activity was 8-fold higher in stem cell-enriched cell populations compared with differentiated cells, whereas Notch1 signaling activity was 4-fold lower in the stem cell-enriched cell populations. Pharmacologic or genetic inhibition of Notch1 or Notch4 reduced stem cell activity in vitro and reduced tumor formation in vivo, but Notch4 inhibition produced a more robust effect with a complete inhibition of tumor initiation observed. Our findings suggest that Notch4-targeted therapies will be more effective than targeting Notch1 in suppressing breast cancer recurrence, as it is initiated by breast cancer stem cells.
    Citation
    Regulation of breast cancer stem cell activity by signaling through the Notch4 receptor. 2010, 70 (2):709-18 Cancer Res.
    Journal
    Cancer Research
    URI
    http://hdl.handle.net/10541/109287
    DOI
    10.1158/0008-5472.CAN-09-1681
    PubMed ID
    20068161
    Type
    Article
    Language
    en
    ISSN
    1538-7445
    ae974a485f413a2113503eed53cd6c53
    10.1158/0008-5472.CAN-09-1681
    Scopus Count
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    All Christie Publications
    All Paterson Institute for Cancer Research
    Medical Oncology

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