Simultaneous measurement of cortisol and cortisone in human saliva using liquid chromatography-tandem mass spectrometry: application in basal and stimulated conditions.
Authors
Perogamvros, IliasOwen, Laura J
Newell-Price, John
Ray, David W
Trainer, Peter J
Keevil, Brian G
Affiliation
Department of Endocrinology, The Christie, Manchester, UK. ilias.perogamvros@nhs.netIssue Date
2009-11-01
Metadata
Show full item recordAbstract
Immunoassays used for the measurement of salivary cortisol are limited by variable interference from cortisone. Salivary cortisone is a consequence of the salivary glands expressing 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2) which converts cortisol to cortisone. We report a combined salivary cortisol and cortisone (SalF and SalE respectively) liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay to address the cortisone cross-reactivity in cortisol immunoassays and as a tool to study 11beta-HSD2 activity. The method was linear up to 400 nmol/L for SalF and 200 nmol/L for SalE and the lower limits of quantitation were 0.39 nmol/L (SalF) and 0.78 nmol/L (SalE). No evidence of ion suppression was found and precision, accuracy and recovery were within internationally accepted limits. No interference was identified from 13 structurally related steroids. SalF, SalE and SalF/SalE were significantly greater in the morning than at bed-time and following stimulation of the adrenal glands. As serum cortisol increased, an exponential rise was observed in SalF and a linear increase in SalE which reached a plateau at higher SalF concentrations. We have developed a novel, robust LC-MS/MS assay for the combined measurement of SalF and SalE. Our results confirm the 11beta-HSD2 activity of the salivary glands resulting in high SalE concentrations and the enzyme saturation at high substrate concentrations. This method can be used as a simple, non-invasive and highly specific tool to assess the value of salivary cortisol as a surrogate for free serum cortisol and as a potential novel way to assess 11beta-HSD2 activity.Citation
Simultaneous measurement of cortisol and cortisone in human saliva using liquid chromatography-tandem mass spectrometry: application in basal and stimulated conditions. 2009, 877 (29):3771-5 J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.Journal
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life SciencesDOI
10.1016/j.jchromb.2009.09.014PubMed ID
19783236Type
ArticleLanguage
enISSN
1873-376Xae974a485f413a2113503eed53cd6c53
10.1016/j.jchromb.2009.09.014
Scopus Count
Related articles
- Simultaneous analysis of cortisol and cortisone in saliva using XLC-MS/MS for fully automated online solid phase extraction.
- Authors: Jones RL, Owen LJ, Adaway JE, Keevil BG
- Issue date: 2012 Jan 15
- Salivary cortisone is a potential biomarker for serum free cortisol.
- Authors: Perogamvros I, Keevil BG, Ray DW, Trainer PJ
- Issue date: 2010 Nov
- Measurement of salivary cortisol with liquid chromatography-tandem mass spectrometry in patients undergoing dynamic endocrine testing.
- Authors: Perogamvros I, Owen LJ, Keevil BG, Brabant G, Trainer PJ
- Issue date: 2010 Jan
- Measurement of urinary free cortisol using liquid chromatography-tandem mass spectrometry: comparison with the urine adapted ACS:180 serum cortisol chemiluminescent immunoassay and development of a new reference range.
- Authors: McCann SJ, Gillingwater S, Keevil BG
- Issue date: 2005 Mar
- Validation of an LC-MS/MS salivary assay for glucocorticoid status assessment: Evaluation of the diurnal fluctuation of cortisol and cortisone and of their association within and between serum and saliva.
- Authors: Mezzullo M, Fanelli F, Fazzini A, Gambineri A, Vicennati V, Di Dalmazi G, Pelusi C, Mazza R, Pagotto U, Pasquali R
- Issue date: 2016 Oct