Cell death induced by vincristine in the intestinal crypts of mice and in a human Burkitt's lymphoma cell line.
AffiliationDepartment of Pathology, University of Queensland Medical School, Herston, Brisbane, Australia.
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AbstractAlthough vincristine is widely used clinically in the treatment of some human cancers, its mechanism of action has not been clearly established. In this study, the patterns of cell death induced by vincristine in the intestinal crypts of mice and in a human Burkitt's lymphoma cell line were investigated by light and electron microscopy. Vincristine was found to enhance apoptosis of interphase cells in both systems and also to cause the arrest of cells in mitosis, the latter effect being more pronounced in the intestinal crypts. Arrested mitotic cells went on to die by a process that had a number of features in common with apoptosis. These include compaction of chromatin (following coalescence of chromosomes), condensation of the cytoplasm, initial preservation of organelle integrity, and eventually the fragmentation of the cell into a number of membrane-enclosed bodies which are morphologically similar to conventional apoptotic bodies. The results suggest that the cytocidal effect of vincristine is not solely dependent on metaphase arrest but is a cumulative one, resulting both from apoptosis of interphase cells and the 'apoptotic-like' death of cells arrested in metaphase.
CitationCell death induced by vincristine in the intestinal crypts of mice and in a human Burkitt's lymphoma cell line. 1992, 25 (6):523-36 Cell Prolif.
- The nature of cytotoxic drug-induced cell death in murine intestinal crypts.
- Authors: Anilkumar TV, Sarraf CE, Hunt T, Alison MR
- Issue date: 1992 Apr
- Variation in the duration of mitosis in the crypts of Lieberkuhn of the rat; a cytokinetic study using vincristine.
- Authors: Wright N, Morley A, Appleton D
- Issue date: 1972 Jul
- Disruption of p53 function in immortalized human cells does not affect survival or apoptosis after taxol or vincristine treatment.
- Authors: Fan S, Cherney B, Reinhold W, Rucker K, O'Connor PM
- Issue date: 1998 Apr
- Demonstration of vincristine resistance in primary intestinal neoplasms in the rat by the 'post-metaphase index'.
- Authors: Ince P, Finney KJ, Appleton DR, Sunter JP, Watson AJ
- Issue date: 1985 Oct
- Cell fate after mitotic arrest in different tumor cells is determined by the balance between slippage and apoptotic threshold.
- Authors: Galán-Malo P, Vela L, Gonzalo O, Calvo-Sanjuán R, Gracia-Fleta L, Naval J, Marzo I
- Issue date: 2012 Feb 1