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dc.contributor.authorBadawi, Alaa Fen
dc.contributor.authorMostafa, M Hen
dc.contributor.authorAboul-Azm, Ten
dc.contributor.authorHaboubi, N Yen
dc.contributor.authorO'Connor, Peter Jen
dc.contributor.authorCooper, Donald Pen
dc.date.accessioned2010-08-04T09:52:11Z
dc.date.available2010-08-04T09:52:11Z
dc.date.issued1992-05
dc.identifier.citationPromutagenic methylation damage in bladder DNA from patients with bladder cancer associated with schistosomiasis and from normal individuals. 1992, 13 (5):877-81 Carcinogenesisen
dc.identifier.issn0143-3334
dc.identifier.pmid1587002
dc.identifier.doi10.1093/carcin/13.5.877
dc.identifier.urihttp://hdl.handle.net/10541/109026
dc.description.abstractRadioimmunoassays (RIAs) have been used to detect the promutagenic lesion O6-methyldeoxyguanosine (O6-MedG) in DNA isolated from the bladder tissues of Egyptian patients presenting with bladder carcinoma and concomitant schistosomiasis (bilharziasis), a parasitic disease known to be associated with the presence of N-nitrosamines in the urine. Alkylation damage was present in the DNA of the majority of the samples (44/46, 96%); 38 samples were of tumour tissue and 8 from uninvolved bladder mucosa. Levels of O6-MedG ranged from undetectable (ND; i.e. less than 0.01 mumol O6-MedG/mol dG) to 0.485 mumol/mol dG with an overall mean of 0.134 +/- 0.10 mumol/mol dG, including the two samples that were below the limit of detection. In contrast, methylation damage was detected in only 4/12 (33%) of the DNA samples from normal bladder tissue of European origin. In these samples levels of O6-MedG ranged from ND to 0.225 mumol/mol dG with an overall mean of 0.046 +/- 0.082 mumol O6-MedG/mol dG. These results confirm that alkylation events can be detected in the DNA of schistosome-infected human bladder tissue. The methylation of uninvolved and tumour tissue DNA to similar extents suggests that the alkylating intermediate may have been present in the urine. These results indicate the need for further investigation to determine whether relationships exist between levels of DNA damage and the prevalence of schistosome infection and/or the extent and type of bacterial infection that frequently accompanies this disease.
dc.language.isoenen
dc.subjectCancer DNAen
dc.subjectUrinary Bladder Canceren
dc.subject.meshAge Factors
dc.subject.meshDNA Damage
dc.subject.meshDNA, Neoplasm
dc.subject.meshDeoxyguanosine
dc.subject.meshEgypt
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMethylation
dc.subject.meshSchistosomiasis haematobia
dc.subject.meshSex Factors
dc.subject.meshUrinary Bladder Neoplasms
dc.titlePromutagenic methylation damage in bladder DNA from patients with bladder cancer associated with schistosomiasis and from normal individuals.en
dc.typeArticleen
dc.contributor.departmentInstitute for Graduate Studies and Research, University of Alexandria, Egypt.en
dc.identifier.journalCarcinogenesisen
html.description.abstractRadioimmunoassays (RIAs) have been used to detect the promutagenic lesion O6-methyldeoxyguanosine (O6-MedG) in DNA isolated from the bladder tissues of Egyptian patients presenting with bladder carcinoma and concomitant schistosomiasis (bilharziasis), a parasitic disease known to be associated with the presence of N-nitrosamines in the urine. Alkylation damage was present in the DNA of the majority of the samples (44/46, 96%); 38 samples were of tumour tissue and 8 from uninvolved bladder mucosa. Levels of O6-MedG ranged from undetectable (ND; i.e. less than 0.01 mumol O6-MedG/mol dG) to 0.485 mumol/mol dG with an overall mean of 0.134 +/- 0.10 mumol/mol dG, including the two samples that were below the limit of detection. In contrast, methylation damage was detected in only 4/12 (33%) of the DNA samples from normal bladder tissue of European origin. In these samples levels of O6-MedG ranged from ND to 0.225 mumol/mol dG with an overall mean of 0.046 +/- 0.082 mumol O6-MedG/mol dG. These results confirm that alkylation events can be detected in the DNA of schistosome-infected human bladder tissue. The methylation of uninvolved and tumour tissue DNA to similar extents suggests that the alkylating intermediate may have been present in the urine. These results indicate the need for further investigation to determine whether relationships exist between levels of DNA damage and the prevalence of schistosome infection and/or the extent and type of bacterial infection that frequently accompanies this disease.


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