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dc.contributor.authorGhosh, Anna K
dc.contributor.authorMoore, M
dc.date.accessioned2010-08-04T09:21:12Z
dc.date.available2010-08-04T09:21:12Z
dc.date.issued1992
dc.identifier.citationTumour-infiltrating lymphocytes in cervical carcinoma. 1992, 28A (11):1910-6 Eur. J. Canceren
dc.identifier.issn0959-8049
dc.identifier.pmid1389534
dc.identifier.doi10.1016/0959-8049(92)90034-Y
dc.identifier.urihttp://hdl.handle.net/10541/109020
dc.description.abstractTumour-infiltrating lymphocytes from cervical carcinomas were cultivated in the presence of interleukin-2. The majority of bulk cultures were cytotoxic against K562, Mel 1 and Caski cells. CD8+ cells were the predominant subset in over 50% of cultures, with varying numbers of CD56+ and CD25+ cells. T cell clones were established from six tumour-infiltrating lymphocyte cultures and the majority exhibited non-MHC-restricted cytotoxicity. However, in one case cytotoxicity of several derived clones was limited to the autologous tumour and in another, to the autologous tumour and Caski cells. This study indicates that tumour-infiltrating lymphocytes can be amplified and cloned from cervical carcinoma biopsies in the presence of rIL2. Although the predominant cytolytic function is non-MHC-restricted, low autotumour cytotoxicity can be demonstrated at the clonal level. The nature of the antigen(s) recognised by T cells on autologous cervical carcinoma cells in unknown; the candidacy of human papillomavirus-related products requires investigation.
dc.language.isoenen
dc.subjectTumour-Infiltrating Lymphocytesen
dc.subjectUterine Cervical Canceren
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshCD4-CD8 Ratio
dc.subject.meshCells, Cultured
dc.subject.meshCytotoxicity, Immunologic
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshInterleukin-2
dc.subject.meshLymphocytes, Tumor-Infiltrating
dc.subject.meshMiddle Aged
dc.subject.meshRecombinant Proteins
dc.subject.meshT-Lymphocytes
dc.subject.meshUterine Cervical Neoplasms
dc.titleTumour-infiltrating lymphocytes in cervical carcinoma.en
dc.typeArticleen
dc.contributor.departmentDepartment of Immunology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, U.K.en
dc.identifier.journalEuropean Journal of Canceren
html.description.abstractTumour-infiltrating lymphocytes from cervical carcinomas were cultivated in the presence of interleukin-2. The majority of bulk cultures were cytotoxic against K562, Mel 1 and Caski cells. CD8+ cells were the predominant subset in over 50% of cultures, with varying numbers of CD56+ and CD25+ cells. T cell clones were established from six tumour-infiltrating lymphocyte cultures and the majority exhibited non-MHC-restricted cytotoxicity. However, in one case cytotoxicity of several derived clones was limited to the autologous tumour and in another, to the autologous tumour and Caski cells. This study indicates that tumour-infiltrating lymphocytes can be amplified and cloned from cervical carcinoma biopsies in the presence of rIL2. Although the predominant cytolytic function is non-MHC-restricted, low autotumour cytotoxicity can be demonstrated at the clonal level. The nature of the antigen(s) recognised by T cells on autologous cervical carcinoma cells in unknown; the candidacy of human papillomavirus-related products requires investigation.


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