A potential pitfall in the use of electroporation: cellular radiosensitization by pulsed high-voltage electric fields.
Authors
West, Catharine M LAffiliation
Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.Issue Date
1992-03
Metadata
Show full item recordAbstract
Chinese hamster ovary cells have been exposed to high-voltage electric fields causing electroporation (EP) and the interaction between EP and radiation-induced cell lethality investigated. There was a voltage-dependent decrease in plating efficiency, assessed immediately following EP, and cell viability, assessed at 24 h. A linear decrease was seen for both. These decreases were accompanied by a voltage-dependent increase in cell volume, assessed immediately following EP. A good correlation between increases in cell volume and decreases in plating efficiency was seen (r = -0.91). The application of electric fields immediately prior to, or following, irradiation led to a radiosensitization of the cells. This radiosensitization still occurred when a 6 h interval was left between radiation and EP but was lost when cells were irradiated 24 h prior to EP. When cells were irradiated following EP, the radiosensitization was lost with a 1 h interval between the two treatments. These results suggest that, when studying the combined cellular effects of EP of macromolecules and radiation, care should be taken that sufficient time has elapsed between the two modalities to prevent the radiosensitization of cells.Citation
A potential pitfall in the use of electroporation: cellular radiosensitization by pulsed high-voltage electric fields. 1992, 61 (3):329-34 Int. J. Radiat. Biol.Journal
International Journal of Radiation BiologyDOI
10.1080/09553009214551011PubMed ID
1347065Type
ArticleLanguage
enISSN
0955-3002ae974a485f413a2113503eed53cd6c53
10.1080/09553009214551011
Scopus Count
Collections
Related articles
- Hyperthermic killing and hyperthermic radiosensitization in Chinese hamster ovary cells: effects of pH and thermal tolerance.
- Authors: Holahan EV, Highfield DP, Holahan PK, Dewey WC
- Issue date: 1984 Jan
- Hyperthermic radiosensitization of thermotolerant Chinese hamster ovary cells.
- Authors: Holahan PK, Wong RS, Thompson LL, Dewey WC
- Issue date: 1986 Sep
- Single Intense Microsecond Electric Pulse Induces Radiosensitization to Ionizing Radiation: Effects of Time Intervals Between Electric Pulse and Ionizing Irradiation.
- Authors: Rezaee Z, Yadollahpour A, Bayati V
- Issue date: 2018
- Permeabilization of the nuclear envelope following nanosecond pulsed electric field exposure.
- Authors: Thompson GL, Roth CC, Kuipers MA, Tolstykh GP, Beier HT, Ibey BL
- Issue date: 2016 Jan 29
- Effect of thermotolerance and step-down heating on thermal radiosensitization in CHO cells.
- Authors: Dikomey E, Jung H
- Issue date: 1992 Feb