Show simple item record

dc.contributor.authorKleibl, K
dc.contributor.authorMargison, Geoffrey P
dc.contributor.authorSkorvaga, M
dc.contributor.authorBrozmanová, J
dc.contributor.authorMasek, F
dc.date.accessioned2010-08-03T11:57:57Z
dc.date.available2010-08-03T11:57:57Z
dc.date.issued1992-05
dc.identifier.citationA recA-ada hybrid gene inducible by DNA damage. 1992, 282 (1):39-42 Mutat. Res.en
dc.identifier.issn0027-5107
dc.identifier.pmid1374156
dc.identifier.urihttp://hdl.handle.net/10541/108947
dc.description.abstractA damage-inducible expression vector was constructed in which the original recA structural gene was replaced by the protein-coding region of the ada gene. The O6-alkylguanine-DNA alkyltransferase encoded by the ada gene can be measured by a rapid and highly sensitive assay. The introduction of this construct into an appropriate host cell provides an effective bacterial assay for genotoxins.
dc.language.isoenen
dc.subject.meshCloning, Molecular
dc.subject.meshDNA Damage
dc.subject.meshEscherichia coli
dc.subject.meshGenetic Vectors
dc.subject.meshMethyltransferases
dc.subject.meshO(6)-Methylguanine-DNA Methyltransferase
dc.subject.meshPlasmids
dc.subject.meshRec A Recombinases
dc.subject.meshRegulatory Sequences, Nucleic Acid
dc.subject.meshSOS Response (Genetics)
dc.titleA recA-ada hybrid gene inducible by DNA damage.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular Genetics, Slovak Academy of Sciences, Bratislava, Czechoslovakia.en
dc.identifier.journalMutation Researchen
html.description.abstractA damage-inducible expression vector was constructed in which the original recA structural gene was replaced by the protein-coding region of the ada gene. The O6-alkylguanine-DNA alkyltransferase encoded by the ada gene can be measured by a rapid and highly sensitive assay. The introduction of this construct into an appropriate host cell provides an effective bacterial assay for genotoxins.


This item appears in the following Collection(s)

Show simple item record