AffiliationDepartment of Molecular Genetics, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
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AbstractA damage-inducible expression vector was constructed in which the original recA structural gene was replaced by the protein-coding region of the ada gene. The O6-alkylguanine-DNA alkyltransferase encoded by the ada gene can be measured by a rapid and highly sensitive assay. The introduction of this construct into an appropriate host cell provides an effective bacterial assay for genotoxins.
CitationA recA-ada hybrid gene inducible by DNA damage. 1992, 282 (1):39-42 Mutat. Res.
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- Authors: Dumenco LL, Warman B, Hatzoglou M, Lim IK, Abboud SL, Gerson SL
- Issue date: 1989 Nov 1
- The recA gene of Chlamydia trachomatis: cloning, sequence, and characterization in Escherichia coli.
- Authors: Hintz NJ, Ennis DG, Liu WF, Larsen SH
- Issue date: 1995 Apr 1
- High level, regulated expression of the chimeric P-enolpyruvate carboxykinase (GTP)-bacterial O6-alkylguanine-DNA alkyltransferase (ada) gene in transgenic mice.
- Authors: Lim IK, Dumenco LL, Yun J, Donovan C, Warman B, Gorodetzkaya N, Wagner TE, Clapp DW, Hanson RW, Gerson SL
- Issue date: 1990 Mar 15
- Cloning and expression of the Bacillus subtilis methyltransferase gene in Escherichia coli ada- cells.
- Authors: Kodama KI, Nakabeppu Y, Sekiguchi M
- Issue date: 1989 Sep
- Efficient repair of hydrogen peroxide-induced DNA damage by Escherichia coli requires SOS induction of RecA and RuvA proteins.
- Authors: Konola JT, Sargent KE, Gow JB
- Issue date: 2000 Apr 28