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dc.contributor.authorFord, A M
dc.contributor.authorBennett, C A
dc.contributor.authorHealy, L E
dc.contributor.authorNavarro, E
dc.contributor.authorSpooncer, Elaine
dc.contributor.authorGreaves, M F
dc.date.accessioned2010-08-03T11:53:57Z
dc.date.available2010-08-03T11:53:57Z
dc.date.issued1992-04-15
dc.identifier.citationImmunoglobulin heavy-chain and CD3 delta-chain gene enhancers are DNase I-hypersensitive in hemopoietic progenitor cells. 1992, 89 (8):3424-8 Proc. Natl. Acad. Sci. U.S.A.en
dc.identifier.issn0027-8424
dc.identifier.pmid1533043
dc.identifier.urihttp://hdl.handle.net/10541/108945
dc.description.abstractMultipotential interleukin 3-dependent non-immortalized murine hemopoietic progenitor cells have DNase I-hypersensitive sites in the immunoglobulin heavy-chain and CD3 delta enhancers and transcribe germ-line T-cell antigen receptor gamma-chain (TCR gamma), but not IgM or TCR beta, genes. Induction of myeloid differentiation in these cells clones down expression and/or transcriptional accessibility of the immunoglobulin heavy-chain and TCR gamma genes. The CD3 delta enhancer region remains DNase I-hypersensitive but closes down in B cells. In embryonic stem cells and pan-mesodermal cells, these genes or enhancer regions are neither expressed nor DNase I-hypersensitive. These data suggest that lineage potential may be programmed, at least in part, by alterations in the accessibility or conformation of regulatory regions of genes and that some promiscuity of gene expression and/or accessibility can precede lineage commitment and maturation in progenitor cells induced to self-renew by interleukin 3.
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals
dc.subject.meshAntigens, CD3
dc.subject.meshAntigens, Differentiation, T-Lymphocyte
dc.subject.meshBone Marrow
dc.subject.meshCell Differentiation
dc.subject.meshCell Line, Transformed
dc.subject.meshCell Nucleus
dc.subject.meshCells, Cultured
dc.subject.meshDeoxyribonuclease I
dc.subject.meshEnhancer Elements, Genetic
dc.subject.meshGene Expression
dc.subject.meshGenes, Immunoglobulin
dc.subject.meshGranulocytes
dc.subject.meshGrowth Substances
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshImmunoglobulin Heavy Chains
dc.subject.meshInterleukin-3
dc.subject.meshMethylation
dc.subject.meshMice
dc.subject.meshReceptors, Antigen, T-Cell
dc.subject.meshRestriction Mapping
dc.subject.meshT-Lymphocytes
dc.subject.meshTranscription, Genetic
dc.titleImmunoglobulin heavy-chain and CD3 delta-chain gene enhancers are DNase I-hypersensitive in hemopoietic progenitor cells.en
dc.typeArticleen
dc.contributor.departmentLeukaemia Research Fund Centre, Chester Beatty Laboratories, London, United Kingdom.en
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of Americaen
html.description.abstractMultipotential interleukin 3-dependent non-immortalized murine hemopoietic progenitor cells have DNase I-hypersensitive sites in the immunoglobulin heavy-chain and CD3 delta enhancers and transcribe germ-line T-cell antigen receptor gamma-chain (TCR gamma), but not IgM or TCR beta, genes. Induction of myeloid differentiation in these cells clones down expression and/or transcriptional accessibility of the immunoglobulin heavy-chain and TCR gamma genes. The CD3 delta enhancer region remains DNase I-hypersensitive but closes down in B cells. In embryonic stem cells and pan-mesodermal cells, these genes or enhancer regions are neither expressed nor DNase I-hypersensitive. These data suggest that lineage potential may be programmed, at least in part, by alterations in the accessibility or conformation of regulatory regions of genes and that some promiscuity of gene expression and/or accessibility can precede lineage commitment and maturation in progenitor cells induced to self-renew by interleukin 3.


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