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dc.contributor.authorHampson, Ian Nen
dc.contributor.authorCross, Michael Aen
dc.contributor.authorHeyworth, Clare Men
dc.contributor.authorFairbairn, Leslie Jen
dc.contributor.authorSpooncer, Elaineen
dc.contributor.authorCowling, Graham Jen
dc.contributor.authorDexter, T Michaelen
dc.date.accessioned2010-08-03T11:05:03Z
dc.date.available2010-08-03T11:05:03Z
dc.date.issued1992-12-15
dc.identifier.citationExpression and downregulation of cytotoxic cell protease 1 or Granzyme 'B' transcripts during myeloid differentiation of interleukin-3-dependent murine stem cell lines. 1992, 80 (12):3097-105 Blooden
dc.identifier.issn0006-4971
dc.identifier.pmid1281690
dc.identifier.urihttp://hdl.handle.net/10541/108933
dc.description.abstractUsing the technique of differential cDNA library screening, we have molecularly cloned a gene that is highly expressed in an undifferentiated myeloid multipotent and growth factor-dependent stem cell line (FDCP-Mix) and that downregulates as these cells are induced to differentiate along monocytic, granulocytic, and erythroid cell lineages. Sequence analysis of this gene has shown homology with a previously cloned gene, cytotoxic cell protease 1 (CCP1 or Granzyme 'B'), that has been shown to be expressed only in thymocytes, activated T cells, a mast cell line, and peritoneal exudate leukocytes. In situ hybridization, Northern blot analysis, and nuclear run-off assay has confirmed that expression of CCP1 is restricted to the phenotypically primitive multipotent undifferentiated. FDCP-Mix cells that are undergoing self-renewal in the presence of growth factors such as interleukin-3.
dc.language.isoenen
dc.subject.meshAmino Acid Sequence
dc.subject.meshAnimals
dc.subject.meshBase Sequence
dc.subject.meshCell Differentiation
dc.subject.meshCell Line
dc.subject.meshCloning, Molecular
dc.subject.meshGene Expression Regulation, Enzymologic
dc.subject.meshGene Library
dc.subject.meshGranulocyte Colony-Stimulating Factor
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor
dc.subject.meshGranzymes
dc.subject.meshGrowth Substances
dc.subject.meshInterleukin-3
dc.subject.meshInterleukin-6
dc.subject.meshMacrophage Colony-Stimulating Factor
dc.subject.meshMice
dc.subject.meshMolecular Sequence Data
dc.subject.meshOpen Reading Frames
dc.subject.meshRNA
dc.subject.meshRecombinant Proteins
dc.subject.meshSerine Endopeptidases
dc.subject.meshStem Cells
dc.subject.meshTranscription, Genetic
dc.subject.meshTransforming Growth Factor beta
dc.titleExpression and downregulation of cytotoxic cell protease 1 or Granzyme 'B' transcripts during myeloid differentiation of interleukin-3-dependent murine stem cell lines.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Experimental Hematology, Paterson Institute for Cancer Research, Withington, Manchester, UK.en
dc.identifier.journalBlooden
html.description.abstractUsing the technique of differential cDNA library screening, we have molecularly cloned a gene that is highly expressed in an undifferentiated myeloid multipotent and growth factor-dependent stem cell line (FDCP-Mix) and that downregulates as these cells are induced to differentiate along monocytic, granulocytic, and erythroid cell lineages. Sequence analysis of this gene has shown homology with a previously cloned gene, cytotoxic cell protease 1 (CCP1 or Granzyme 'B'), that has been shown to be expressed only in thymocytes, activated T cells, a mast cell line, and peritoneal exudate leukocytes. In situ hybridization, Northern blot analysis, and nuclear run-off assay has confirmed that expression of CCP1 is restricted to the phenotypically primitive multipotent undifferentiated. FDCP-Mix cells that are undergoing self-renewal in the presence of growth factors such as interleukin-3.


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