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    Expression and downregulation of cytotoxic cell protease 1 or Granzyme 'B' transcripts during myeloid differentiation of interleukin-3-dependent murine stem cell lines.

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    Authors
    Hampson, Ian N
    Cross, Michael A
    Heyworth, Clare M
    Fairbairn, Leslie J
    Spooncer, Elaine
    Cowling, Graham J
    Dexter, T Michael
    Affiliation
    Cancer Research Campaign Department of Experimental Hematology, Paterson Institute for Cancer Research, Withington, Manchester, UK.
    Issue Date
    1992-12-15
    
    Metadata
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    Abstract
    Using the technique of differential cDNA library screening, we have molecularly cloned a gene that is highly expressed in an undifferentiated myeloid multipotent and growth factor-dependent stem cell line (FDCP-Mix) and that downregulates as these cells are induced to differentiate along monocytic, granulocytic, and erythroid cell lineages. Sequence analysis of this gene has shown homology with a previously cloned gene, cytotoxic cell protease 1 (CCP1 or Granzyme 'B'), that has been shown to be expressed only in thymocytes, activated T cells, a mast cell line, and peritoneal exudate leukocytes. In situ hybridization, Northern blot analysis, and nuclear run-off assay has confirmed that expression of CCP1 is restricted to the phenotypically primitive multipotent undifferentiated. FDCP-Mix cells that are undergoing self-renewal in the presence of growth factors such as interleukin-3.
    Citation
    Expression and downregulation of cytotoxic cell protease 1 or Granzyme 'B' transcripts during myeloid differentiation of interleukin-3-dependent murine stem cell lines. 1992, 80 (12):3097-105 Blood
    Journal
    Blood
    URI
    http://hdl.handle.net/10541/108933
    PubMed ID
    1281690
    Type
    Article
    Language
    en
    ISSN
    0006-4971
    Collections
    All Paterson Institute for Cancer Research

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