Show simple item record

dc.contributor.authorWest, Catharine M L
dc.contributor.authorMoore, James V
dc.date.accessioned2010-08-03T08:32:37Z
dc.date.available2010-08-03T08:32:37Z
dc.date.issued1992-03
dc.identifier.citationMechanisms behind the resistance of spheroids to photodynamic treatment: a flow cytometry study. 1992, 55 (3):425-30 Photochem. Photobiol.en
dc.identifier.issn0031-8655
dc.identifier.pmid1532855
dc.identifier.doi10.1111/j.1751-1097.1992.tb04257.x
dc.identifier.urihttp://hdl.handle.net/10541/108896
dc.description.abstractThe influence of cell heterogeneity on response to photodynamic treatment (PDT) has been investigated using the human colon adenocarcinoma line WiDr, grown as spheroids and exposed to hematoporphyrin derivative. The spheroids show a marked spheroid size-dependent resistance to PDT. Using a flow cytometer, cell sub-populations have been separated, on the basis of drug fluorescence, from single cell suspensions prepared from 500 microm diameter spheroids. Cells low in fluorescence have been shown to be resistant to PDT, have a smaller median cell volume, and be enhanced in G1-type cells. These cells also show reduced low density lipoprotein uptake. The results suggest that spheroid size-dependent resistance to PDT is related to a decreasing growth fraction with increasing spheroid size. Heterogeneity of drug uptake could be a potential limitation to clinical PDT.
dc.language.isoenen
dc.subjectHaematoporphyrinsen
dc.subjectHaematoporphyrin Derivativeen
dc.subjectCultured Tumour Cellsen
dc.subject.meshAdenocarcinoma
dc.subject.meshAntineoplastic Agents
dc.subject.meshBiological Transport, Active
dc.subject.meshColonic Neoplasms
dc.subject.meshDrug Resistance
dc.subject.meshFlow Cytometry
dc.subject.meshHematoporphyrin Derivative
dc.subject.meshHematoporphyrins
dc.subject.meshHumans
dc.subject.meshPhotochemotherapy
dc.subject.meshRadiation-Sensitizing Agents
dc.subject.meshTumor Cells, Cultured
dc.titleMechanisms behind the resistance of spheroids to photodynamic treatment: a flow cytometry study.en
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, U.K.en
dc.identifier.journalPhotochemistry and Photobiologyen
html.description.abstractThe influence of cell heterogeneity on response to photodynamic treatment (PDT) has been investigated using the human colon adenocarcinoma line WiDr, grown as spheroids and exposed to hematoporphyrin derivative. The spheroids show a marked spheroid size-dependent resistance to PDT. Using a flow cytometer, cell sub-populations have been separated, on the basis of drug fluorescence, from single cell suspensions prepared from 500 microm diameter spheroids. Cells low in fluorescence have been shown to be resistant to PDT, have a smaller median cell volume, and be enhanced in G1-type cells. These cells also show reduced low density lipoprotein uptake. The results suggest that spheroid size-dependent resistance to PDT is related to a decreasing growth fraction with increasing spheroid size. Heterogeneity of drug uptake could be a potential limitation to clinical PDT.


This item appears in the following Collection(s)

Show simple item record