Mechanisms behind the resistance of spheroids to photodynamic treatment: a flow cytometry study.
dc.contributor.author | West, Catharine M L | |
dc.contributor.author | Moore, James V | |
dc.date.accessioned | 2010-08-03T08:32:37Z | |
dc.date.available | 2010-08-03T08:32:37Z | |
dc.date.issued | 1992-03 | |
dc.identifier.citation | Mechanisms behind the resistance of spheroids to photodynamic treatment: a flow cytometry study. 1992, 55 (3):425-30 Photochem. Photobiol. | en |
dc.identifier.issn | 0031-8655 | |
dc.identifier.pmid | 1532855 | |
dc.identifier.doi | 10.1111/j.1751-1097.1992.tb04257.x | |
dc.identifier.uri | http://hdl.handle.net/10541/108896 | |
dc.description.abstract | The influence of cell heterogeneity on response to photodynamic treatment (PDT) has been investigated using the human colon adenocarcinoma line WiDr, grown as spheroids and exposed to hematoporphyrin derivative. The spheroids show a marked spheroid size-dependent resistance to PDT. Using a flow cytometer, cell sub-populations have been separated, on the basis of drug fluorescence, from single cell suspensions prepared from 500 microm diameter spheroids. Cells low in fluorescence have been shown to be resistant to PDT, have a smaller median cell volume, and be enhanced in G1-type cells. These cells also show reduced low density lipoprotein uptake. The results suggest that spheroid size-dependent resistance to PDT is related to a decreasing growth fraction with increasing spheroid size. Heterogeneity of drug uptake could be a potential limitation to clinical PDT. | |
dc.language.iso | en | en |
dc.subject | Haematoporphyrins | en |
dc.subject | Haematoporphyrin Derivative | en |
dc.subject | Cultured Tumour Cells | en |
dc.subject.mesh | Adenocarcinoma | |
dc.subject.mesh | Antineoplastic Agents | |
dc.subject.mesh | Biological Transport, Active | |
dc.subject.mesh | Colonic Neoplasms | |
dc.subject.mesh | Drug Resistance | |
dc.subject.mesh | Flow Cytometry | |
dc.subject.mesh | Hematoporphyrin Derivative | |
dc.subject.mesh | Hematoporphyrins | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Photochemotherapy | |
dc.subject.mesh | Radiation-Sensitizing Agents | |
dc.subject.mesh | Tumor Cells, Cultured | |
dc.title | Mechanisms behind the resistance of spheroids to photodynamic treatment: a flow cytometry study. | en |
dc.type | Article | en |
dc.contributor.department | Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, U.K. | en |
dc.identifier.journal | Photochemistry and Photobiology | en |
html.description.abstract | The influence of cell heterogeneity on response to photodynamic treatment (PDT) has been investigated using the human colon adenocarcinoma line WiDr, grown as spheroids and exposed to hematoporphyrin derivative. The spheroids show a marked spheroid size-dependent resistance to PDT. Using a flow cytometer, cell sub-populations have been separated, on the basis of drug fluorescence, from single cell suspensions prepared from 500 microm diameter spheroids. Cells low in fluorescence have been shown to be resistant to PDT, have a smaller median cell volume, and be enhanced in G1-type cells. These cells also show reduced low density lipoprotein uptake. The results suggest that spheroid size-dependent resistance to PDT is related to a decreasing growth fraction with increasing spheroid size. Heterogeneity of drug uptake could be a potential limitation to clinical PDT. |