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    Mechanisms behind the resistance of spheroids to photodynamic treatment: a flow cytometry study.

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    Authors
    West, Catharine M L
    Moore, James V
    Affiliation
    Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, U.K.
    Issue Date
    1992-03
    
    Metadata
    Show full item record
    Abstract
    The influence of cell heterogeneity on response to photodynamic treatment (PDT) has been investigated using the human colon adenocarcinoma line WiDr, grown as spheroids and exposed to hematoporphyrin derivative. The spheroids show a marked spheroid size-dependent resistance to PDT. Using a flow cytometer, cell sub-populations have been separated, on the basis of drug fluorescence, from single cell suspensions prepared from 500 microm diameter spheroids. Cells low in fluorescence have been shown to be resistant to PDT, have a smaller median cell volume, and be enhanced in G1-type cells. These cells also show reduced low density lipoprotein uptake. The results suggest that spheroid size-dependent resistance to PDT is related to a decreasing growth fraction with increasing spheroid size. Heterogeneity of drug uptake could be a potential limitation to clinical PDT.
    Citation
    Mechanisms behind the resistance of spheroids to photodynamic treatment: a flow cytometry study. 1992, 55 (3):425-30 Photochem. Photobiol.
    Journal
    Photochemistry and Photobiology
    URI
    http://hdl.handle.net/10541/108896
    DOI
    10.1111/j.1751-1097.1992.tb04257.x
    PubMed ID
    1532855
    Type
    Article
    Language
    en
    ISSN
    0031-8655
    ae974a485f413a2113503eed53cd6c53
    10.1111/j.1751-1097.1992.tb04257.x
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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