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dc.contributor.authorBaker, Bartrum W
dc.contributor.authorNorton, John D
dc.date.accessioned2010-08-03T08:56:44Z
dc.date.available2010-08-03T08:56:44Z
dc.date.issued1992
dc.identifier.citationAnalysis of mutations in the Gs protein alpha subunit gene in human leukaemia. 1992, 16 (5):485-9 Leuk. Res.en
dc.identifier.issn0145-2126
dc.identifier.pmid1625474
dc.identifier.doi10.1016/0145-2126(92)90174-6
dc.identifier.urihttp://hdl.handle.net/10541/108887
dc.description.abstractMutations at codons 201 and 227 that cause oncogenic activation of the gene encoding the Gs alpha subunit protein occur in a subset of human solid tumours. To determine whether such oncogenic mutations occur in leukaemia we have analysed DNA from leukaemic cells from 59 patients representing a spectrum of acute and chronic leukaemia types by direct nucleotide sequence analysis of polymerase chain reaction (PCR) amplified DNA. No mutations were detected at oncogenic hot spots represented by codons 201 or 227, nor in any other codons extending from nucleotide position 196-230 in the Gs alpha subunit gene. This strongly suggests that this region of the Gs alpha gene is highly nonpolymorphic and that oncogenic mutations in this gene are unlikely to play a significant role in the pathogenesis of leukaemia.
dc.language.isoenen
dc.subjectLeukaemiaen
dc.subject.meshAmino Acid Sequence
dc.subject.meshCodon
dc.subject.meshDNA Mutational Analysis
dc.subject.meshGTP-Binding Proteins
dc.subject.meshHumans
dc.subject.meshLeukemia
dc.subject.meshMolecular Sequence Data
dc.subject.meshMutation
dc.subject.meshPolymerase Chain Reaction
dc.titleAnalysis of mutations in the Gs protein alpha subunit gene in human leukaemia.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Gene Regulation, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, U.K.en
dc.identifier.journalLeukemia Researchen
html.description.abstractMutations at codons 201 and 227 that cause oncogenic activation of the gene encoding the Gs alpha subunit protein occur in a subset of human solid tumours. To determine whether such oncogenic mutations occur in leukaemia we have analysed DNA from leukaemic cells from 59 patients representing a spectrum of acute and chronic leukaemia types by direct nucleotide sequence analysis of polymerase chain reaction (PCR) amplified DNA. No mutations were detected at oncogenic hot spots represented by codons 201 or 227, nor in any other codons extending from nucleotide position 196-230 in the Gs alpha subunit gene. This strongly suggests that this region of the Gs alpha gene is highly nonpolymorphic and that oncogenic mutations in this gene are unlikely to play a significant role in the pathogenesis of leukaemia.


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