Growth of human umbilical-cord blood in longterm haemopoietic cultures.
dc.contributor.author | Hows, Jill M | |
dc.contributor.author | Bradley, B A | |
dc.contributor.author | Marsh, Judith C W | |
dc.contributor.author | Luft, Thomas | |
dc.contributor.author | Coutinho, Lucia H | |
dc.contributor.author | Testa, Nydia G | |
dc.contributor.author | Dexter, T Michael | |
dc.date.accessioned | 2010-08-03T08:55:33Z | |
dc.date.available | 2010-08-03T08:55:33Z | |
dc.date.issued | 1992-07-11 | |
dc.identifier.citation | Growth of human umbilical-cord blood in longterm haemopoietic cultures. 1992, 340 (8811):73-6 Lancet | en |
dc.identifier.issn | 0140-6736 | |
dc.identifier.pmid | 1352013 | |
dc.identifier.doi | 10.1016/0140-6736(92)90396-K | |
dc.identifier.uri | http://hdl.handle.net/10541/108886 | |
dc.description.abstract | Cryopreserved human umbilical-cord (HUC) blood is an alternative to bone marrow as a source of haemopoietic "stem" cells for HLA-identical transplantation of children with leukaemia or Fanconi's anaemia. We have studied the in-vitro growth potential of HUC blood in clonogenic assays and in longterm haemopoietic cultures. Clonogenic assays showed that HUC blood produced as many haemopoietic-cell colonies as normal adult bone marrow and a higher proportion of primitive-cell colonies. In longterm culture on preformed irradiated marrow stroma, both progenitor-cell production and lifespan of cultures were significantly greater in HUC blood than in normal bone marrow (p = 0.0007). Our findings indicate that the quality and quantity of HUC-blood-derived haemopoietic "stem" cells are better than those of normal bone marrow. Therefore, single HUC-blood donations are probably sufficient for adults requiring transplantation for leukaemia and other haemopoietic disorders. Banking of HLA-typed HUC blood to facilitate transplantation of patients who lack a family donor should be considered. | |
dc.language.iso | en | en |
dc.subject | Haematopoietic Stem Cell Transplantation | en |
dc.subject | Haematopoietic Stem Cells | en |
dc.subject.mesh | Blood Transfusion | |
dc.subject.mesh | Bone Marrow | |
dc.subject.mesh | Cell Division | |
dc.subject.mesh | Cell Survival | |
dc.subject.mesh | Cells, Cultured | |
dc.subject.mesh | Colony-Forming Units Assay | |
dc.subject.mesh | Cryopreservation | |
dc.subject.mesh | Erythroid Precursor Cells | |
dc.subject.mesh | Evaluation Studies as Topic | |
dc.subject.mesh | Fetal Blood | |
dc.subject.mesh | Granulocyte-Macrophage Colony-Stimulating Factor | |
dc.subject.mesh | Hematopoietic Stem Cell Transplantation | |
dc.subject.mesh | Hematopoietic Stem Cells | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Time Factors | |
dc.title | Growth of human umbilical-cord blood in longterm haemopoietic cultures. | en |
dc.type | Article | en |
dc.contributor.department | Kay Kendall Laboratory, Paterson Institute, Cancer Research Campaign, Manchester, UK. | en |
dc.identifier.journal | Lancet | en |
html.description.abstract | Cryopreserved human umbilical-cord (HUC) blood is an alternative to bone marrow as a source of haemopoietic "stem" cells for HLA-identical transplantation of children with leukaemia or Fanconi's anaemia. We have studied the in-vitro growth potential of HUC blood in clonogenic assays and in longterm haemopoietic cultures. Clonogenic assays showed that HUC blood produced as many haemopoietic-cell colonies as normal adult bone marrow and a higher proportion of primitive-cell colonies. In longterm culture on preformed irradiated marrow stroma, both progenitor-cell production and lifespan of cultures were significantly greater in HUC blood than in normal bone marrow (p = 0.0007). Our findings indicate that the quality and quantity of HUC-blood-derived haemopoietic "stem" cells are better than those of normal bone marrow. Therefore, single HUC-blood donations are probably sufficient for adults requiring transplantation for leukaemia and other haemopoietic disorders. Banking of HLA-typed HUC blood to facilitate transplantation of patients who lack a family donor should be considered. |