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    Growth of human umbilical-cord blood in longterm haemopoietic cultures.

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    Authors
    Hows, Jill M
    Bradley, B A
    Marsh, Judith C W
    Luft, Thomas
    Coutinho, Lucia H
    Testa, Nydia G
    Dexter, T Michael
    Affiliation
    Kay Kendall Laboratory, Paterson Institute, Cancer Research Campaign, Manchester, UK.
    Issue Date
    1992-07-11
    
    Metadata
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    Abstract
    Cryopreserved human umbilical-cord (HUC) blood is an alternative to bone marrow as a source of haemopoietic "stem" cells for HLA-identical transplantation of children with leukaemia or Fanconi's anaemia. We have studied the in-vitro growth potential of HUC blood in clonogenic assays and in longterm haemopoietic cultures. Clonogenic assays showed that HUC blood produced as many haemopoietic-cell colonies as normal adult bone marrow and a higher proportion of primitive-cell colonies. In longterm culture on preformed irradiated marrow stroma, both progenitor-cell production and lifespan of cultures were significantly greater in HUC blood than in normal bone marrow (p = 0.0007). Our findings indicate that the quality and quantity of HUC-blood-derived haemopoietic "stem" cells are better than those of normal bone marrow. Therefore, single HUC-blood donations are probably sufficient for adults requiring transplantation for leukaemia and other haemopoietic disorders. Banking of HLA-typed HUC blood to facilitate transplantation of patients who lack a family donor should be considered.
    Citation
    Growth of human umbilical-cord blood in longterm haemopoietic cultures. 1992, 340 (8811):73-6 Lancet
    Journal
    Lancet
    URI
    http://hdl.handle.net/10541/108886
    DOI
    10.1016/0140-6736(92)90396-K
    PubMed ID
    1352013
    Type
    Article
    Language
    en
    ISSN
    0140-6736
    ae974a485f413a2113503eed53cd6c53
    10.1016/0140-6736(92)90396-K
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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