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dc.contributor.authorAngelis, K
dc.contributor.authorBríza, J
dc.contributor.authorSatava, J
dc.contributor.authorSkákal, I
dc.contributor.authorVelemínský, J
dc.contributor.authorVlasák, J
dc.contributor.authorKleibl, K
dc.contributor.authorMargison, Geoffrey P
dc.date.accessioned2010-08-03T08:38:45Z
dc.date.available2010-08-03T08:38:45Z
dc.date.issued1992-05
dc.identifier.citationIncreased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada. 1992, 273 (3):271-80 Mutat. Res.en
dc.identifier.issn0027-5107
dc.identifier.pmid1374848
dc.identifier.doi10.1016/0921-8777(92)90089-L
dc.identifier.urihttp://hdl.handle.net/10541/108881
dc.description.abstractThe protein coding region of the E. coli gene ada has been transferred to tobacco plants by a leaf disc transformation procedure involving an Agrobacterium tumefaciens Ti plasmid. Transformed plants were shown to be transgenic for the ada message and had increased levels of O6-alkylguanine DNA alkyltransferase activity. The N-methyl-N-nitrosourea- or taurinechlorethylnitrosourea-induced inhibition of growth of calluses or of cells in suspension was considerably lower in ada-transformed than in non-transformed plants. This indicates that O6-alkylguanine, O4-alkylthymine or phosphotriesters are growth-inhibitory lesions in tobacco.
dc.language.isoenen
dc.subject.meshAlkylating Agents
dc.subject.meshBlotting, Southern
dc.subject.meshCloning, Molecular
dc.subject.meshDNA Repair
dc.subject.meshEscherichia coli
dc.subject.meshGenes, Bacterial
dc.subject.meshMethyltransferases
dc.subject.meshO(6)-Methylguanine-DNA Methyltransferase
dc.subject.meshPlants, Toxic
dc.subject.meshPlasmids
dc.subject.meshRhizobium radiobacter
dc.subject.meshTobacco
dc.subject.meshTransfection
dc.titleIncreased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada.en
dc.typeArticleen
dc.contributor.departmentInstitute of Experimental Botany CSAV, Prague.en
dc.identifier.journalMutation Researchen
html.description.abstractThe protein coding region of the E. coli gene ada has been transferred to tobacco plants by a leaf disc transformation procedure involving an Agrobacterium tumefaciens Ti plasmid. Transformed plants were shown to be transgenic for the ada message and had increased levels of O6-alkylguanine DNA alkyltransferase activity. The N-methyl-N-nitrosourea- or taurinechlorethylnitrosourea-induced inhibition of growth of calluses or of cells in suspension was considerably lower in ada-transformed than in non-transformed plants. This indicates that O6-alkylguanine, O4-alkylthymine or phosphotriesters are growth-inhibitory lesions in tobacco.


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