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    Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada.

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    Authors
    Angelis, K
    Bríza, J
    Satava, J
    Skákal, I
    Velemínský, J
    Vlasák, J
    Kleibl, K
    Margison, Geoffrey P
    Affiliation
    Institute of Experimental Botany CSAV, Prague.
    Issue Date
    1992-05
    
    Metadata
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    Abstract
    The protein coding region of the E. coli gene ada has been transferred to tobacco plants by a leaf disc transformation procedure involving an Agrobacterium tumefaciens Ti plasmid. Transformed plants were shown to be transgenic for the ada message and had increased levels of O6-alkylguanine DNA alkyltransferase activity. The N-methyl-N-nitrosourea- or taurinechlorethylnitrosourea-induced inhibition of growth of calluses or of cells in suspension was considerably lower in ada-transformed than in non-transformed plants. This indicates that O6-alkylguanine, O4-alkylthymine or phosphotriesters are growth-inhibitory lesions in tobacco.
    Citation
    Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada. 1992, 273 (3):271-80 Mutat. Res.
    Journal
    Mutation Research
    URI
    http://hdl.handle.net/10541/108881
    DOI
    10.1016/0921-8777(92)90089-L
    PubMed ID
    1374848
    Type
    Article
    Language
    en
    ISSN
    0027-5107
    ae974a485f413a2113503eed53cd6c53
    10.1016/0921-8777(92)90089-L
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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