Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada.
AffiliationInstitute of Experimental Botany CSAV, Prague.
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AbstractThe protein coding region of the E. coli gene ada has been transferred to tobacco plants by a leaf disc transformation procedure involving an Agrobacterium tumefaciens Ti plasmid. Transformed plants were shown to be transgenic for the ada message and had increased levels of O6-alkylguanine DNA alkyltransferase activity. The N-methyl-N-nitrosourea- or taurinechlorethylnitrosourea-induced inhibition of growth of calluses or of cells in suspension was considerably lower in ada-transformed than in non-transformed plants. This indicates that O6-alkylguanine, O4-alkylthymine or phosphotriesters are growth-inhibitory lesions in tobacco.
CitationIncreased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada. 1992, 273 (3):271-80 Mutat. Res.