Inducible alkyltransferase DNA repair proteins in the filamentous fungus Aspergillus nidulans.
| dc.contributor.author | Baker, S M | |
| dc.contributor.author | Margison, Geoffrey P | |
| dc.contributor.author | Strike, P | |
| dc.date.accessioned | 2010-08-03T08:35:47Z | |
| dc.date.available | 2010-08-03T08:35:47Z | |
| dc.date.issued | 1992-02-25 | |
| dc.identifier.citation | Inducible alkyltransferase DNA repair proteins in the filamentous fungus Aspergillus nidulans. 1992, 20 (4):645-51 Nucleic Acids Res. | en |
| dc.identifier.issn | 0305-1048 | |
| dc.identifier.pmid | 1542560 | |
| dc.identifier.doi | 10.1093/nar/20.4.645 | |
| dc.identifier.uri | http://hdl.handle.net/10541/108879 | |
| dc.description.abstract | We have investigated the response of the filamentous fungus Aspergillus nidulans to low, non-killing, doses of the alkylating agent MNNG (N-methyl-N'-nitro-N-nitrosoguanidine). Such treatment causes a substantial induction of DNA alkyltransferase activity, with the specific activity in treated cells increasing up to one hundred-fold. Fluorography reveals the two main inducible species as proteins of 18.5 kDa and 21 kDa, both of which have activity primarily against O6-methylguanine (O6-MeG) lesions. In addition, two other alkyltransferase proteins can also be detected. One, of MW 16 kDa, is expressed in non-treated cells, but is not induced to the same extent as the 18.5 and 21 kDa proteins. The other, a protein of 19.5 kDa, is highly inducible and can only be detected in treated cells. Unlike the other three proteins, it acts primarily against methyl-phosphotriester (Me-PT) lesions. This is the first instance in which an MePT alkyltransferase has been detected in a eukaryotic organism and, coupled with the high level of induction of the O6-MeG alkyltransferase enzymes, this indicates that a control system similar to the bacterial adaptive response may be present in filamentous fungi. | |
| dc.language.iso | en | en |
| dc.subject.mesh | Alkyl and Aryl Transferases | |
| dc.subject.mesh | Aspergillus nidulans | |
| dc.subject.mesh | DNA Repair | |
| dc.subject.mesh | DNA, Fungal | |
| dc.subject.mesh | Electrophoresis | |
| dc.subject.mesh | Enzyme Induction | |
| dc.subject.mesh | Methylnitronitrosoguanidine | |
| dc.subject.mesh | Methyltransferases | |
| dc.subject.mesh | O(6)-Methylguanine-DNA Methyltransferase | |
| dc.subject.mesh | Transferases | |
| dc.title | Inducible alkyltransferase DNA repair proteins in the filamentous fungus Aspergillus nidulans. | en |
| dc.type | Article | en |
| dc.contributor.department | Department of Genetics and Microbiology, University of Liverpool, UK. | en |
| dc.identifier.journal | Nucleic Acids Research | en |
| html.description.abstract | We have investigated the response of the filamentous fungus Aspergillus nidulans to low, non-killing, doses of the alkylating agent MNNG (N-methyl-N'-nitro-N-nitrosoguanidine). Such treatment causes a substantial induction of DNA alkyltransferase activity, with the specific activity in treated cells increasing up to one hundred-fold. Fluorography reveals the two main inducible species as proteins of 18.5 kDa and 21 kDa, both of which have activity primarily against O6-methylguanine (O6-MeG) lesions. In addition, two other alkyltransferase proteins can also be detected. One, of MW 16 kDa, is expressed in non-treated cells, but is not induced to the same extent as the 18.5 and 21 kDa proteins. The other, a protein of 19.5 kDa, is highly inducible and can only be detected in treated cells. Unlike the other three proteins, it acts primarily against methyl-phosphotriester (Me-PT) lesions. This is the first instance in which an MePT alkyltransferase has been detected in a eukaryotic organism and, coupled with the high level of induction of the O6-MeG alkyltransferase enzymes, this indicates that a control system similar to the bacterial adaptive response may be present in filamentous fungi. |