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dc.contributor.authorBaker, S Men
dc.contributor.authorMargison, Geoffrey Pen
dc.contributor.authorStrike, Pen
dc.date.accessioned2010-08-03T08:35:47Z
dc.date.available2010-08-03T08:35:47Z
dc.date.issued1992-02-25
dc.identifier.citationInducible alkyltransferase DNA repair proteins in the filamentous fungus Aspergillus nidulans. 1992, 20 (4):645-51 Nucleic Acids Res.en
dc.identifier.issn0305-1048
dc.identifier.pmid1542560
dc.identifier.doi10.1093/nar/20.4.645
dc.identifier.urihttp://hdl.handle.net/10541/108879
dc.description.abstractWe have investigated the response of the filamentous fungus Aspergillus nidulans to low, non-killing, doses of the alkylating agent MNNG (N-methyl-N'-nitro-N-nitrosoguanidine). Such treatment causes a substantial induction of DNA alkyltransferase activity, with the specific activity in treated cells increasing up to one hundred-fold. Fluorography reveals the two main inducible species as proteins of 18.5 kDa and 21 kDa, both of which have activity primarily against O6-methylguanine (O6-MeG) lesions. In addition, two other alkyltransferase proteins can also be detected. One, of MW 16 kDa, is expressed in non-treated cells, but is not induced to the same extent as the 18.5 and 21 kDa proteins. The other, a protein of 19.5 kDa, is highly inducible and can only be detected in treated cells. Unlike the other three proteins, it acts primarily against methyl-phosphotriester (Me-PT) lesions. This is the first instance in which an MePT alkyltransferase has been detected in a eukaryotic organism and, coupled with the high level of induction of the O6-MeG alkyltransferase enzymes, this indicates that a control system similar to the bacterial adaptive response may be present in filamentous fungi.
dc.language.isoenen
dc.subject.meshAlkyl and Aryl Transferases
dc.subject.meshAspergillus nidulans
dc.subject.meshDNA Repair
dc.subject.meshDNA, Fungal
dc.subject.meshElectrophoresis
dc.subject.meshEnzyme Induction
dc.subject.meshMethylnitronitrosoguanidine
dc.subject.meshMethyltransferases
dc.subject.meshO(6)-Methylguanine-DNA Methyltransferase
dc.subject.meshTransferases
dc.titleInducible alkyltransferase DNA repair proteins in the filamentous fungus Aspergillus nidulans.en
dc.typeArticleen
dc.contributor.departmentDepartment of Genetics and Microbiology, University of Liverpool, UK.en
dc.identifier.journalNucleic Acids Researchen
html.description.abstractWe have investigated the response of the filamentous fungus Aspergillus nidulans to low, non-killing, doses of the alkylating agent MNNG (N-methyl-N'-nitro-N-nitrosoguanidine). Such treatment causes a substantial induction of DNA alkyltransferase activity, with the specific activity in treated cells increasing up to one hundred-fold. Fluorography reveals the two main inducible species as proteins of 18.5 kDa and 21 kDa, both of which have activity primarily against O6-methylguanine (O6-MeG) lesions. In addition, two other alkyltransferase proteins can also be detected. One, of MW 16 kDa, is expressed in non-treated cells, but is not induced to the same extent as the 18.5 and 21 kDa proteins. The other, a protein of 19.5 kDa, is highly inducible and can only be detected in treated cells. Unlike the other three proteins, it acts primarily against methyl-phosphotriester (Me-PT) lesions. This is the first instance in which an MePT alkyltransferase has been detected in a eukaryotic organism and, coupled with the high level of induction of the O6-MeG alkyltransferase enzymes, this indicates that a control system similar to the bacterial adaptive response may be present in filamentous fungi.


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