Inducible alkyltransferase DNA repair proteins in the filamentous fungus Aspergillus nidulans.
AffiliationDepartment of Genetics and Microbiology, University of Liverpool, UK.
MetadataShow full item record
AbstractWe have investigated the response of the filamentous fungus Aspergillus nidulans to low, non-killing, doses of the alkylating agent MNNG (N-methyl-N'-nitro-N-nitrosoguanidine). Such treatment causes a substantial induction of DNA alkyltransferase activity, with the specific activity in treated cells increasing up to one hundred-fold. Fluorography reveals the two main inducible species as proteins of 18.5 kDa and 21 kDa, both of which have activity primarily against O6-methylguanine (O6-MeG) lesions. In addition, two other alkyltransferase proteins can also be detected. One, of MW 16 kDa, is expressed in non-treated cells, but is not induced to the same extent as the 18.5 and 21 kDa proteins. The other, a protein of 19.5 kDa, is highly inducible and can only be detected in treated cells. Unlike the other three proteins, it acts primarily against methyl-phosphotriester (Me-PT) lesions. This is the first instance in which an MePT alkyltransferase has been detected in a eukaryotic organism and, coupled with the high level of induction of the O6-MeG alkyltransferase enzymes, this indicates that a control system similar to the bacterial adaptive response may be present in filamentous fungi.
CitationInducible alkyltransferase DNA repair proteins in the filamentous fungus Aspergillus nidulans. 1992, 20 (4):645-51 Nucleic Acids Res.
JournalNucleic Acids Research
- X-ray induction of O6-alkylguanine-DNA alkyltransferase protects against some of the biological effects of N-methyl-N'-nitro-N-nitrosoguanidine in C3H 10T1/2 cells.
- Authors: von Hofe E, Kennedy AR
- Issue date: 1991 Aug
- The bacterial alkyltransferase-like (eATL) protein protects mammalian cells against methylating agent-induced toxicity.
- Authors: Tomaszowski KH, Aasland D, Margison GP, Williams E, Pinder SI, Modesti M, Fuchs RP, Kaina B
- Issue date: 2015 Apr
- Relationship between O6-alkylguanine-DNA alkyltransferase activity and N-methyl-N'-nitro-N-nitrosoguanidine-induced mutation, transformation, and cytotoxicity in C3H/10T1/2 cells expressing exogenous alkyltransferase genes.
- Authors: von Hofe E, Fairbairn L, Margison GP
- Issue date: 1992 Dec 1
- Induction of c-fos, c-jun, junB and junD mRNA and AP-1 by alkylating mutagens in cells deficient and proficient for the DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) and its relationship to cell death, mutation induction and chromosomal instability.
- Authors: Dosch J, Kaina B
- Issue date: 1996 Nov 7
- Repair of alkylation damage in the fungus Aspergillus nidulans.
- Authors: Swirski RA, Shawcross SG, Faulkner BM, Strike P
- Issue date: 1988 May