Further characterization of monoclonal antibody indicates specificity for (6-4)-dipyrimidine photoproducts.
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Johns Hopkins School of Hygiene and Public Health, Baltimore, MD 21205.Issue Date
1992-05
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Monoclonal antibody aUVssDNA-1 is produced by hybridoma cell line 25JF.C3B6 originally selected from cell fusions using spleen cells from mice immunized with UV-irradiated polydeoxynucleotides (Strickland and Boyle, Photochem. Photobiol. 34, 595-601, 1981). Original and subsequent studies of the binding characteristics of aUVssDNA-1 indicated that it was specific for cyclobuta-dithymidine photoproducts. Those investigations examined action spectrum, short-wavelength photo-reversal, nucleotide sequence effects, and photoreactivation using E. coli photolyase and incandescent light. However, the more recent studies reported here examined acetophenone-UV-B photosensitization, UV-B photoisomerization, and photoreactivation using cloned E. coli photolyase and filtered incandescent light. The results indicate that aUVssDNA-1 recognizes photoproducts with characteristics of (6-4)-dipyrimidines. Thus, previous studies in which relatively rapid repair of cyclobuta-dithymidine photoproducts was inferred using this antibody, require re-interpretation in light of these new findings.Citation
Further characterization of monoclonal antibody indicates specificity for (6-4)-dipyrimidine photoproducts. 1992, 55 (5):723-7 Photochem. Photobiol.Journal
Photochemistry and PhotobiologyDOI
10.1111/j.1751-1097.1992.tb08516.xPubMed ID
1528986Type
ArticleLanguage
enISSN
0031-8655ae974a485f413a2113503eed53cd6c53
10.1111/j.1751-1097.1992.tb08516.x
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