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dc.contributor.authorBoughdady, I S
dc.contributor.authorKinsella, Anne R
dc.contributor.authorHaboubi, N Y
dc.contributor.authorSchofield, Philip F
dc.date.accessioned2010-08-03T08:25:29Z
dc.date.available2010-08-03T08:25:29Z
dc.date.issued1992-08
dc.identifier.citationK-ras gene mutations in adenomas and carcinomas of the colon. 1992, 1 (4):275-82 Surg Oncolen
dc.identifier.issn0960-7404
dc.identifier.pmid1341261
dc.identifier.doi10.1016/0960-7404(92)90088-3
dc.identifier.urihttp://hdl.handle.net/10541/108877
dc.description.abstractDNA extracted from 29 colorectal carcinomas and 40 sporadic adenomas was amplified by the polymerase chain reaction (PCR) and analysed for the presence of K-ras gene mutations at codon 12 using a panel of synthetic oligonucleotide probes specific for normal and mutated sequences. The presence of mutations was correlated with various histopathological and clinical data. Ten carcinomas (34.5%) and 14 sporadic adenomas (35%) showed K-ras mutations at codon 12. In the carcinoma group, no apparent correlation was found between the presence of mutant oncogenes and the degree of histological differentiation, Dukes' staging or the development of distant metastasis. In the adenoma group, the frequency of mutations increased with the size of the adenoma and the severity of the dysplastic changes. This study confirms that ras gene mutations are common and early events in colon carcinogenesis. They appear to give a selective growth advantage to those polyps with mutations which leads to their increase in size and thus possibly prepare the ground for malignant transformation.
dc.language.isoenen
dc.subjectColonic Canceren
dc.subject.meshAdenoma
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshBase Sequence
dc.subject.meshCarcinoma
dc.subject.meshCodon
dc.subject.meshColonic Neoplasms
dc.subject.meshColonic Polyps
dc.subject.meshDNA
dc.subject.meshFemale
dc.subject.meshGenes, ras
dc.subject.meshHumans
dc.subject.meshImmunoblotting
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshMolecular Sequence Data
dc.subject.meshMutation
dc.subject.meshNucleic Acid Hybridization
dc.subject.meshOligonucleotide Probes
dc.subject.meshPolymerase Chain Reaction
dc.titleK-ras gene mutations in adenomas and carcinomas of the colon.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalSurgical Oncologyen
html.description.abstractDNA extracted from 29 colorectal carcinomas and 40 sporadic adenomas was amplified by the polymerase chain reaction (PCR) and analysed for the presence of K-ras gene mutations at codon 12 using a panel of synthetic oligonucleotide probes specific for normal and mutated sequences. The presence of mutations was correlated with various histopathological and clinical data. Ten carcinomas (34.5%) and 14 sporadic adenomas (35%) showed K-ras mutations at codon 12. In the carcinoma group, no apparent correlation was found between the presence of mutant oncogenes and the degree of histological differentiation, Dukes' staging or the development of distant metastasis. In the adenoma group, the frequency of mutations increased with the size of the adenoma and the severity of the dysplastic changes. This study confirms that ras gene mutations are common and early events in colon carcinogenesis. They appear to give a selective growth advantage to those polyps with mutations which leads to their increase in size and thus possibly prepare the ground for malignant transformation.


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