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dc.contributor.authorBoughdady, I S
dc.contributor.authorKinsella, Anne R
dc.contributor.authorHaboubi, N Y
dc.contributor.authorSchofield, Philip F
dc.date.accessioned2010-08-03T08:22:08Z
dc.date.available2010-08-03T08:22:08Z
dc.date.issued1992-08
dc.identifier.citationK-ras gene mutation in colorectal adenomas and carcinomas from familial adenomatous polyposis patients. 1992, 1 (4):269-74 Surg Oncolen
dc.identifier.issn0960-7404
dc.identifier.pmid1341260
dc.identifier.doi10.1016/0960-7404(92)90087-2
dc.identifier.urihttp://hdl.handle.net/10541/108876
dc.description.abstractColorectal adenomas and carcinomas from familial adenomatous polyposis (FAP) patients were screened for the presence of K-ras gene mutations at codon 12 using an in vitro amplification step (polymerase chain reaction) followed by dot blot analysis using oligonucleotide probes specific for different mutations at codon 12. We examined 28 colorectal adenomas and two colorectal carcinomas from 12 FAP patients and observed a mutation at codon 12 in seven adenomas and in both carcinomas. The frequency of K-ras gene mutations in colorectal tumours from FAP patients is similar to those in cases of sporadic adenomas and sporadic colorectal carcinomas indicating that the mechanisms involved in their development may be similar.
dc.language.isoenen
dc.subjectColorectal Canceren
dc.subject.meshAdenoma
dc.subject.meshAdenomatous Polyposis Coli
dc.subject.meshAdult
dc.subject.meshBase Sequence
dc.subject.meshCarcinoma
dc.subject.meshChild
dc.subject.meshCodon
dc.subject.meshColorectal Neoplasms
dc.subject.meshDNA
dc.subject.meshFemale
dc.subject.meshGenes, ras
dc.subject.meshHumans
dc.subject.meshImmunoblotting
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshMolecular Sequence Data
dc.subject.meshMutation
dc.subject.meshNucleic Acid Hybridization
dc.subject.meshOligonucleotide Probes
dc.subject.meshPolymerase Chain Reaction
dc.titleK-ras gene mutation in colorectal adenomas and carcinomas from familial adenomatous polyposis patients.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalSurgical Oncologyen
html.description.abstractColorectal adenomas and carcinomas from familial adenomatous polyposis (FAP) patients were screened for the presence of K-ras gene mutations at codon 12 using an in vitro amplification step (polymerase chain reaction) followed by dot blot analysis using oligonucleotide probes specific for different mutations at codon 12. We examined 28 colorectal adenomas and two colorectal carcinomas from 12 FAP patients and observed a mutation at codon 12 in seven adenomas and in both carcinomas. The frequency of K-ras gene mutations in colorectal tumours from FAP patients is similar to those in cases of sporadic adenomas and sporadic colorectal carcinomas indicating that the mechanisms involved in their development may be similar.


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