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    Effect of enkephalins on bone marrow cells.

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    Authors
    Krizanac-Bengez, L
    Boranic, M
    Testa, Nydia G
    Marotti, T
    Affiliation
    Department of Experimental Biology and Medicine, Ruder Boskovic Institute, Zagreb, Croatia.
    Issue Date
    1992
    
    Metadata
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    Abstract
    Mouse bone marrow cells were incubated with methionine- or leucine-enkephalin (10(-15)-10(-6) M) before seeding into soft agar cultures. In marrow samples harvested at different times, enkephalins decreased GM colony count on average by 30-40%. In individual experiments, however, the same concentration of enkephalins caused even stimulation, or at other times had effect. In view of the circadian periodicity of neuroendocrine functions and hematopoietic activity, the enkephalin effect on bone marrow cells was tested on marrow samples harvested at fixed time points (6 am, 6 pm), using enkephalin concentrations in the physiological range (10(-12)-10(-9) M). The seeding efficiency of the 6-pm cell population was on average 50% above that of the 6-am population. The 6-pm cell population was also more susceptible to the inhibitory effect of the enkephalins (35% inhibition) than the 6-am population (15% inhibition), and the variability in response was considerably reduced. With progenitor cell-enriched population, obtained by fluorescence-activated cell sorting (FACS) of 6-am bone marrow samples, in 3 out of 6 experiments Met- and Leu-enkephalin showed 30-35% inhibition of GM colony formation over a wide range of concentrations (10(-15)-10(-6)). In the other 3 experiments, suppression as well as stimulation or no alteration in colony count were observed. This variability probably reflected quality (purity) of the progenitor cell population, and may indicate that the enkephalins affected hematopoietic cells via a population of accessory cells.
    Citation
    Effect of enkephalins on bone marrow cells. 1992, 46 (8):367-73 Biomed. Pharmacother.
    Journal
    Biomedicine & Pharmacotherapy
    URI
    http://hdl.handle.net/10541/108846
    DOI
    10.1016/0753-3322(92)90305-Q
    PubMed ID
    1292647
    Type
    Article
    Language
    en
    ISSN
    0753-3322
    ae974a485f413a2113503eed53cd6c53
    10.1016/0753-3322(92)90305-Q
    Scopus Count
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