Multilineage phenotypes of interleukin-3-dependent progenitor cells.
AffiliationLeukaemia Research Fund Centre, Chester Beatty Laboratories, London, England.
MetadataShow full item record
AbstractInterleukin-3 (IL-3)-dependent murine FDCP-mix cells have multilineage differentiation capacity; they are nonleukemic, have a normal karyotype, and are nonimmortalized. These cells coexpress on their cell surface the "early" B-lineage marker B220/CD45R and the myeloid marker Mac-1/iC3b receptor (CR3), transcribe germline T-cell receptor gamma genes, and express the macrophage lineage growth factor receptor gene c-fms as a predominant 8.4-kb transcript. They do not detectably express at the stable mRNA or protein level other lymphoid precursor cell genes including CD2, TdT, lambda 5, and BP1. Induction of granulocyte/macrophage differentiation in these cells closes down expression of the lymphoid genes and activates stable expression of genes specific to the myeloid lineage, including myeloperoxidase. Expression of the c-fms gene at the mRNA level is upregulated and the dominant stable transcript is now in the 4.1-kb form typical of the macrophage lineage. These data provide a plausible explanation for the coexpression of lymphoid and myeloid lineage markers on human leukemic cells of stem cell or progenitor cell origin and have implications for the programming of lineage potential in normal multipotential hematopoiteic progenitor cells.
CitationMultilineage phenotypes of interleukin-3-dependent progenitor cells. 1992, 79 (8):1962-71 Blood
- Transcriptional regulation of the c-fms proto-oncogene mediated by granulocyte/macrophage colony-stimulating factor (GM-CSF) in murine cell lines.
- Authors: Helftenbein G, Krusekopf K, Just U, Cross M, Ostertag W, Niemann H, Tamura T
- Issue date: 1996 Feb 15
- Flt3 ligand induces the outgrowth of Mac-1+B220+ mouse bone marrow progenitor cells restricted to macrophage differentiation that coexpress early B cell-associated genes.
- Authors: Dannaeus K, Johannisson A, Nilsson K, Jönsson JI
- Issue date: 1999 Nov
- Focal adhesion kinase upregulated by granulocyte-macrophage colony-stimulating factor but not by interleukin-3 in differentiating myeloid cells.
- Authors: Kume A, Nishiura H, Suda J, Suda T
- Issue date: 1997 May 1
- Recombinant interleukin 3 induces interleukin 2 receptor expression on early myeloid cells in normal human bone marrow.
- Authors: Gazzola MV, Collins NH, Tafuri A, Keever CA
- Issue date: 1992 Feb
- Premature expression of the macrophage colony-stimulating factor receptor on a multipotential stem cell line does not alter differentiation lineages controlled by stromal cells used for coculture.
- Authors: Kinashi T, Lee KH, Ogawa M, Tohyama K, Tashiro K, Fukunaga R, Nagata S, Honjo T
- Issue date: 1991 May 1