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    Follicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: modulation by calcium.

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    Authors
    Talbot, J A
    Lambert, A
    Mitchell, R
    Grabinski, M
    Anderson, D C
    Tsatsoulis, Agathocles
    Shalet, Stephen M
    Robertson, W R
    Affiliation
    Department of Medicine (Clinical Biochemistry), University of Manchester, Hope Hospital, Salford, UK.
    Issue Date
    1991-09
    
    Metadata
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    Abstract
    We have investigated the role of Ca2+ in the control of FSH-induced estradiol secretion by Sertoli cells isolated from 8-10 days old rats. Exogenous Ca2+ (4-8 mmol/l) inhibited FSH-stimulated E2 secretion such that, with 8 mmol/l Ca2+ and FSH (8 IU/l) E2 secretion decreased from 2091 +/- 322 to 1480 +/- 84 pmol/l (p less than 0.002), whilst chelation of Ca2+ in the culture medium with EGTA (3 mmol/l) increased E2 secretion from 360 +/- 45 to 1242 +/- 133 pmol/l) in the absence of FSH. Further, EGTA (3 mmol/l) markedly potentiated FSH (8 IU/l), forskolin (1 mumol/l) and dibutyryl cAMP (1 mmol/l)-stimulated E2 secretion. Addition of the Ca2+ ionophores, ionomycin (2-5 mumol/l) and A23187 (2 mumol/l), inhibited FSH (8 IU/l)-stimulated E2 secretion by greater than 80%. The effect of ionomycin was totally reversible, whereas that of A23187 was irreversible. Ionomycin (5 mumol/l) had no effect on EGTA-induced E2 secretion in the absence of FSH, but reduced EGTA-provoked E2 secretion by 59% in the presence of FSH (8 IU/l). Similarly, forskolin- and dibutyryl cAMP-provoked E2 production was inhibited 46-50% by ionomycin (5 mumol/l). We conclude that FSH-induced E2 secretion from immature rat Sertoli cells is modulated by intra- and extracellular Ca2+.
    Citation
    Follicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: modulation by calcium. 1991, 125 (3):280-5 Acta Endocrinol.
    Journal
    Acta Endocrinologica
    URI
    http://hdl.handle.net/10541/108403
    DOI
    10.1530/acta.0.1250280
    PubMed ID
    1659097
    Type
    Article
    Language
    en
    ISSN
    0001-5598
    ae974a485f413a2113503eed53cd6c53
    10.1530/acta.0.1250280
    Scopus Count
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