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dc.contributor.authorMargison, Jennifer M
dc.contributor.authorWilkinson, Peter M
dc.contributor.authorCerny, T
dc.contributor.authorThatcher, Nick
dc.date.accessioned2010-07-21T10:15:23Z
dc.date.available2010-07-21T10:15:23Z
dc.date.issued1986-06
dc.identifier.citationA simple quantitative HPLC assay for ifosfamide in biological fluids. 1986, 1 (3):101-3 Biomed. Chromatogr.en
dc.identifier.issn0269-3879
dc.identifier.pmid3506819
dc.identifier.doi10.1002/bmc.1130010303
dc.identifier.urihttp://hdl.handle.net/10541/108030
dc.description.abstractA high performance liquid chromatography method is described for measuring Ifosfamide (I) in human serum. This involves solvent extraction, reverse phase HPLC and UV detection at 190 nm. Standard curves of peak height x detector sensitivity versus I concentration in serum were linear with a lower limit of detection of 100 ng/ml. Authentic 14C-labelled I cochromatographed with standard I and with I found in serum from treated patients. The concentration-time curves of I determined by both HPLC and gas chromatography were indistinguishable. We conclude that this method is suitable for determining I pharmacokinetics in biological specimens.
dc.language.isoenen
dc.subject.meshCarbon Radioisotopes
dc.subject.meshChromatography, High Pressure Liquid
dc.subject.meshHumans
dc.subject.meshIfosfamide
dc.subject.meshSpectrophotometry, Ultraviolet
dc.titleA simple quantitative HPLC assay for ifosfamide in biological fluids.en
dc.typeArticleen
dc.contributor.departmentDepartment of Clinical Pharmacology, Christie Hospital, Holt Radium Institute, Manchester, UK.en
dc.identifier.journalBiomedical Chromatographyen
html.description.abstractA high performance liquid chromatography method is described for measuring Ifosfamide (I) in human serum. This involves solvent extraction, reverse phase HPLC and UV detection at 190 nm. Standard curves of peak height x detector sensitivity versus I concentration in serum were linear with a lower limit of detection of 100 ng/ml. Authentic 14C-labelled I cochromatographed with standard I and with I found in serum from treated patients. The concentration-time curves of I determined by both HPLC and gas chromatography were indistinguishable. We conclude that this method is suitable for determining I pharmacokinetics in biological specimens.


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