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dc.contributor.authorDazzi, H
dc.contributor.authorHasleton, Philip S
dc.contributor.authorThatcher, Nick
dc.contributor.authorWilkes, S
dc.contributor.authorBarnes, D
dc.contributor.authorGullick, W J
dc.date.accessioned2010-07-21T08:34:11Z
dc.date.available2010-07-21T08:34:11Z
dc.date.issued1991-03
dc.identifier.citationComparison of epidermal growth factor receptor expression in lung cancer by immunohistochemical staining of both frozen and formalin fixed specimens. 1991, 3 (2):69-75 Anal Cell Patholen
dc.identifier.issn0921-8912
dc.identifier.pmid2025606
dc.identifier.urihttp://hdl.handle.net/10541/107999
dc.description.abstractTwo monoclonal antibodies used to investigate the expression of the epidermal growth factor receptor (EGF-R) in 45 lung cancers were compared. The R1 antibody recognises the extracellular domain portion of the receptor and the F4 is directed against the cytoplasmic portion. The reactivities of the two antibodies have been compared in fresh frozen tumour specimens. In addition the staining activity of the F4 antibody (the first to EGF-R which can be used in archival material) was compared using frozen and paraffin sections from the same series of tumours. Comparisons of the numbers of cells staining with the R1 and F4 antibody showed only slight discrepancy when fresh material was examined. The discrepancy that existed could be explained by the heterogeneity of the tumours. Very similar results were obtained using the F4 antibody on paraffin embedded and fresh non-small cell lung cancer material. We conclude that the expression EGF-R can be detected reliably by the F4 antibody in both the fresh frozen and formalin fixed, paraffin embedded material and could be useful in assessing the clinical importance of EGF-R in archival tumour material.
dc.language.isoenen
dc.subjectLung Canceren
dc.subject.meshCytological Techniques
dc.subject.meshFormaldehyde
dc.subject.meshFreezing
dc.subject.meshHumans
dc.subject.meshLung Neoplasms
dc.subject.meshNitrogen
dc.subject.meshReceptor, Epidermal Growth Factor
dc.titleComparison of epidermal growth factor receptor expression in lung cancer by immunohistochemical staining of both frozen and formalin fixed specimens.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Medical Oncology, Christie Hospital, Manchester, England, UK.en
dc.identifier.journalAnalytical Cellular Pathologyen
html.description.abstractTwo monoclonal antibodies used to investigate the expression of the epidermal growth factor receptor (EGF-R) in 45 lung cancers were compared. The R1 antibody recognises the extracellular domain portion of the receptor and the F4 is directed against the cytoplasmic portion. The reactivities of the two antibodies have been compared in fresh frozen tumour specimens. In addition the staining activity of the F4 antibody (the first to EGF-R which can be used in archival material) was compared using frozen and paraffin sections from the same series of tumours. Comparisons of the numbers of cells staining with the R1 and F4 antibody showed only slight discrepancy when fresh material was examined. The discrepancy that existed could be explained by the heterogeneity of the tumours. Very similar results were obtained using the F4 antibody on paraffin embedded and fresh non-small cell lung cancer material. We conclude that the expression EGF-R can be detected reliably by the F4 antibody in both the fresh frozen and formalin fixed, paraffin embedded material and could be useful in assessing the clinical importance of EGF-R in archival tumour material.


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