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    Comparison of epidermal growth factor receptor expression in lung cancer by immunohistochemical staining of both frozen and formalin fixed specimens.

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    Authors
    Dazzi, H
    Hasleton, Philip S
    Thatcher, Nick
    Wilkes, S
    Barnes, D
    Gullick, W J
    Affiliation
    CRC Department of Medical Oncology, Christie Hospital, Manchester, England, UK.
    Issue Date
    1991-03
    
    Metadata
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    Abstract
    Two monoclonal antibodies used to investigate the expression of the epidermal growth factor receptor (EGF-R) in 45 lung cancers were compared. The R1 antibody recognises the extracellular domain portion of the receptor and the F4 is directed against the cytoplasmic portion. The reactivities of the two antibodies have been compared in fresh frozen tumour specimens. In addition the staining activity of the F4 antibody (the first to EGF-R which can be used in archival material) was compared using frozen and paraffin sections from the same series of tumours. Comparisons of the numbers of cells staining with the R1 and F4 antibody showed only slight discrepancy when fresh material was examined. The discrepancy that existed could be explained by the heterogeneity of the tumours. Very similar results were obtained using the F4 antibody on paraffin embedded and fresh non-small cell lung cancer material. We conclude that the expression EGF-R can be detected reliably by the F4 antibody in both the fresh frozen and formalin fixed, paraffin embedded material and could be useful in assessing the clinical importance of EGF-R in archival tumour material.
    Citation
    Comparison of epidermal growth factor receptor expression in lung cancer by immunohistochemical staining of both frozen and formalin fixed specimens. 1991, 3 (2):69-75 Anal Cell Pathol
    Journal
    Analytical Cellular Pathology
    URI
    http://hdl.handle.net/10541/107999
    PubMed ID
    2025606
    Type
    Article
    Language
    en
    ISSN
    0921-8912
    Collections
    All Christie Publications

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