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dc.contributor.authorRinke de Wit, T F
dc.contributor.authorWilson, L
dc.contributor.authorVan den Elsen, P J
dc.contributor.authorThielen, F
dc.contributor.authorBrekhoff, D
dc.contributor.authorOosterhuis, J W
dc.contributor.authorPera, M F
dc.contributor.authorStern, Peter L
dc.date.accessioned2010-06-21T13:58:26Z
dc.date.available2010-06-21T13:58:26Z
dc.date.issued1991-08
dc.identifier.citationMonoclonal antibodies to human embryonal carcinoma cells: antigenic relationships of germ cell tumors. 1991, 65 (2):180-91 Lab. Invest.en
dc.identifier.issn0023-6837
dc.identifier.pmid1881121
dc.identifier.urihttp://hdl.handle.net/10541/106576
dc.description.abstractFifteen monoclonal antibodies (mAb) that show specificity for human embryonal carcinoma cells are described. C57BL/6 mice were immunized with Tera-2 embryonal carcinoma cells, and hybridomas were isolated and tested versus a set of human developmental tumor cell lines. The antigens exhibit relatively restricted and unique distributions on normal tissues as shown by immunohistochemistry. The mAbs recognize a series of differentiation antigens since their expression changes when teratocarcinoma stem cells are induced to differentiate in vitro. The expression of these molecules in germ cell and related tumors is consistent with the data obtained from in vitro cell studies. Seven of these mAbs immunoprecipitate molecules from surface labeled Tera-2 cells that show distinct molecular weights on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The antigenic phenotypes defined by the set of mAbs can be used to investigate possible pathogenetic relationships of the various testicular tumors.
dc.language.isoenen
dc.subjectCancer Antigensen
dc.subjectCancerous Stem Cellsen
dc.subjectCultured Tumour Cellsen
dc.subject.meshAnimals
dc.subject.meshAntibodies, Monoclonal
dc.subject.meshAntibody Specificity
dc.subject.meshAntigens, Differentiation
dc.subject.meshAntigens, Neoplasm
dc.subject.meshEmbryonal Carcinoma Stem Cells
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshImmunohistochemistry
dc.subject.meshMice
dc.subject.meshMice, Inbred C57BL
dc.subject.meshNeoplastic Stem Cells
dc.subject.meshPrecipitin Tests
dc.subject.meshTumor Cells, Cultured
dc.titleMonoclonal antibodies to human embryonal carcinoma cells: antigenic relationships of germ cell tumors.en
dc.typeArticleen
dc.contributor.departmentDepartment of Immunohematology, Leiden University Hospital, REP-TNO, Rijswijk, The Netherlands.en
dc.identifier.journalLaboratory Investigationen
html.description.abstractFifteen monoclonal antibodies (mAb) that show specificity for human embryonal carcinoma cells are described. C57BL/6 mice were immunized with Tera-2 embryonal carcinoma cells, and hybridomas were isolated and tested versus a set of human developmental tumor cell lines. The antigens exhibit relatively restricted and unique distributions on normal tissues as shown by immunohistochemistry. The mAbs recognize a series of differentiation antigens since their expression changes when teratocarcinoma stem cells are induced to differentiate in vitro. The expression of these molecules in germ cell and related tumors is consistent with the data obtained from in vitro cell studies. Seven of these mAbs immunoprecipitate molecules from surface labeled Tera-2 cells that show distinct molecular weights on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The antigenic phenotypes defined by the set of mAbs can be used to investigate possible pathogenetic relationships of the various testicular tumors.


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