Interleukin-3-dependent hematopoietic stem cell lines capable of osteoclast formation in vitro.
Authors
Hagenaars, C EKawilarang-de Haas, E W
Van der Kraan, A A
Spooncer, Elaine
Dexter, T Michael
Nijweide, P J
Affiliation
Laboratory of Cell Biology and Histology, Leiden, The Netherlands.Issue Date
1991-09
Metadata
Show full item recordAbstract
Recently we reported that the osteoclast originates from the pluripotent hematopoietic stem cell. However, a detailed analysis of the progenitor and precursor stages of the osteoclast lineage is hard to perform with primary cultures of stem cells. In the present investigation interleukin-3 (IL-3)-dependent multipotent hematopoietic stem cell lines (FDCP-mix), which have many characteristics in common with freshly isolated hematopoietic stem cell lines (FDCP-mix), which have many characteristics in common with freshly isolated hematopoietic stem cells, were assayed for their osteoclast formation capacity. FDCP-mix cell lines A4, C2GM, and 15S were cocultured with periosteum-free 17-day-old fetal metatarsal bones. The effects of culture time, medium composition, and addition of WEHI-3b-conditioned medium (an unpurified IL-3 preparation) on osteoclast formation were studied. 15S cells never differentiated into osteoclasts. Both A4 and C2GM cells were able to generate osteoclasts. Osteoclast formation was visualized by staining for tartrate-resistant acid phosphatase activity and confirmed by 45Ca release assays and electron microscopic studies. Medium supplemented with fetal calf serum clearly supported osteoclast formation from A4 cells better than medium supplemented with cock serum. The difference between fetal calf serum and horse serum is generally less pronounced. C2GM cells formed osteoclasts more readily and, generally, earlier than A4 under all culture conditions. WEHI-3b-conditioned medium addition increased the numbers of osteoclasts and their resorption activity. The coculture of stripped metatarsal bones with FDCP-mix cell lines therefore offers a model system with many possibilities for the study of osteoclastogenesis and its regulation.Citation
Interleukin-3-dependent hematopoietic stem cell lines capable of osteoclast formation in vitro. 1991, 6 (9):947-54 J. Bone Miner. Res.Journal
Journal of Bone and Mineral ResearchDOI
10.1002/jbmr.5650060908PubMed ID
1724106Type
ArticleLanguage
enISSN
0884-0431ae974a485f413a2113503eed53cd6c53
10.1002/jbmr.5650060908
Scopus Count
Collections
Related articles
- Murine macrophage precursor cell lines are unable to differentiate into osteoclasts: a possible implication for osteoclast ontogeny.
- Authors: De Grooth R, Mieremet RH, Kawilarang-De Haas EW, Nijweide PJ
- Issue date: 1994 Aug
- Prostaglandin E2 stimulates osteoclast-like cell formation and bone-resorbing activity via osteoblasts: role of cAMP-dependent protein kinase.
- Authors: Kaji H, Sugimoto T, Kanatani M, Fukase M, Kumegawa M, Chihara K
- Issue date: 1996 Jan
- Human trabecular bone-derived osteoblasts support human osteoclast formation in vitro in a defined, serum-free medium.
- Authors: Atkins GJ, Kostakis P, Welldon KJ, Vincent C, Findlay DM, Zannettino AC
- Issue date: 2005 Jun
- Systematic analysis of the ability of stromal cell lines derived from different murine adult tissues to support maintenance of hematopoietic stem cells in vitro.
- Authors: Rios M, Williams DA
- Issue date: 1990 Dec
- Commitment and differentiation of stem cells to the osteoclast lineage.
- Authors: Hayashi S, Yamane T, Miyamoto A, Hemmi H, Tagaya H, Tanio Y, Kanda H, Yamazaki H, Kunisada T
- Issue date: 1998