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dc.contributor.authorWilks, Deepti P
dc.contributor.authorWest, Catharine M L
dc.date.accessioned2010-06-11T11:42:37Z
dc.date.available2010-06-11T11:42:37Z
dc.date.issued1991-11
dc.identifier.citationA serum-free medium for the Courtenay-Mills soft agar assay. 1991, 9 (6):559-69 Int. J. Cell Cloningen
dc.identifier.issn0737-1454
dc.identifier.pmid1770230
dc.identifier.doi10.1002/stem.5530090606
dc.identifier.urihttp://hdl.handle.net/10541/104701
dc.description.abstractA serum-free medium has been developed to support the clonogenic growth in soft agar of human cervical carcinoma cell lines xenografted into nude mice. Using the Courtenay-Mills assay, colony-forming efficiencies and colony sizes equivalent to those obtained using Ham's F12 plus 15% fetal calf serum can be obtained. Validation of the assay using the developed medium was obtained through establishing linearity between the number of cells plated and colonies formed, and by producing radiation survival curves.
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subjectTumour Stem Cell Assayen
dc.subject.meshAgar
dc.subject.meshAnimals
dc.subject.meshCell Survival
dc.subject.meshClone Cells
dc.subject.meshCulture Media, Serum-Free
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshMice, Nude
dc.subject.meshTumor Cells, Cultured
dc.subject.meshTumor Stem Cell Assay
dc.titleA serum-free medium for the Courtenay-Mills soft agar assay.en
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Manchester, United Kingdom.en
dc.identifier.journalInternational Journal of Cell Cloningen
html.description.abstractA serum-free medium has been developed to support the clonogenic growth in soft agar of human cervical carcinoma cell lines xenografted into nude mice. Using the Courtenay-Mills assay, colony-forming efficiencies and colony sizes equivalent to those obtained using Ham's F12 plus 15% fetal calf serum can be obtained. Validation of the assay using the developed medium was obtained through establishing linearity between the number of cells plated and colonies formed, and by producing radiation survival curves.


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