The radiosensitivity of populations of murine hemopoietic colony-forming cells that respond to combinations of growth factors.
dc.contributor.author | Baird, M C | |
dc.contributor.author | Hendry, Jolyon H | |
dc.contributor.author | Dexter, T Michael | |
dc.contributor.author | Testa, Nydia G | |
dc.date.accessioned | 2010-06-11T10:45:51Z | |
dc.date.available | 2010-06-11T10:45:51Z | |
dc.date.issued | 1991-05 | |
dc.identifier.citation | The radiosensitivity of populations of murine hemopoietic colony-forming cells that respond to combinations of growth factors. 1991, 19 (4):282-7 Exp. Hematol. | en |
dc.identifier.issn | 0301-472X | |
dc.identifier.pmid | 2055293 | |
dc.identifier.uri | http://hdl.handle.net/10541/104695 | |
dc.description.abstract | Combinations of murine recombinant interleukin 3 (IL-3), purified murine macrophage colony-stimulating factor (M-CSF), and human recombinant interleukin 1 alpha (IL-1 alpha) were used to determine the effects of growth factors on the measured radiosensitivity of different populations of murine colony-forming cells (CFC). The data showed that combinations of growth factors resulted in different values of CFC radiosensitivity, being less than values observed when colony growth was stimulated using a single factor. For various combinations of growth factors, Do values ranged from 106 +/- 8 to 175 +/- 24 cGy for progenitor cells in normal bone marrow; 74 +/- 3 to 171 +/- 18 cGy for primitive multipotent CFC enriched using fluorescence-activated cell sorting; and from 46 +/- 4 to 131 +/- 10 cGy for more mature granulocyte-macrophage CFC, enriched by counterflow centrifugal elutriation. Only combinations of three factors produced the high values of Do reported in experiments using unpurified conditioned medium as a stimulus for colony formation. | |
dc.language.iso | en | en |
dc.subject.mesh | Animals | |
dc.subject.mesh | Bone Marrow | |
dc.subject.mesh | Drug Combinations | |
dc.subject.mesh | Female | |
dc.subject.mesh | Flow Cytometry | |
dc.subject.mesh | Interleukin-3 | |
dc.subject.mesh | Macrophage Colony-Stimulating Factor | |
dc.subject.mesh | Male | |
dc.subject.mesh | Mice | |
dc.subject.mesh | Radiation Tolerance | |
dc.subject.mesh | Stem Cells | |
dc.title | The radiosensitivity of populations of murine hemopoietic colony-forming cells that respond to combinations of growth factors. | en |
dc.type | Article | en |
dc.contributor.department | Cancer Research Campaign Department of Radiobiology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK. | en |
dc.identifier.journal | Experimental Hematology | en |
html.description.abstract | Combinations of murine recombinant interleukin 3 (IL-3), purified murine macrophage colony-stimulating factor (M-CSF), and human recombinant interleukin 1 alpha (IL-1 alpha) were used to determine the effects of growth factors on the measured radiosensitivity of different populations of murine colony-forming cells (CFC). The data showed that combinations of growth factors resulted in different values of CFC radiosensitivity, being less than values observed when colony growth was stimulated using a single factor. For various combinations of growth factors, Do values ranged from 106 +/- 8 to 175 +/- 24 cGy for progenitor cells in normal bone marrow; 74 +/- 3 to 171 +/- 18 cGy for primitive multipotent CFC enriched using fluorescence-activated cell sorting; and from 46 +/- 4 to 131 +/- 10 cGy for more mature granulocyte-macrophage CFC, enriched by counterflow centrifugal elutriation. Only combinations of three factors produced the high values of Do reported in experiments using unpurified conditioned medium as a stimulus for colony formation. |