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    Cellular interactions in erythroblastic islands in long-term bone marrow cultures, as studied by time-lapse video.

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    Authors
    Allen, Terence D
    Testa, Nydia G
    Affiliation
    Department of Ultrastructure, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.
    Issue Date
    1991
    
    Metadata
    Show full item record
    Abstract
    Long-term bone marrow cultures (LTBMC) are readily converted from the usual granulopoietic to erythropoietic production by the addition of anemic mouse serum (AMS). The "statics" of proliferation and maturation, previously shown by ultrastructural methods to closely mirror the in vivo situation, were studied dynamically using a time-lapse video system. Several cell pedigrees were followed, but the most complete series showed three successive divisions and subsequent enucleations in the progeny of three synchronously mitotic cells observed in the culture; this is indicative of a five division sequence in the erythron. As in erythroblastic islets observed in marrow in vivo, the striking synchrony of maturation was maintained in vitro. Furthermore, when some of the erythroid progeny became displaced to other macrophages, the synchrony, which was maintained by the original erythroid group on the original erythroblastic islet macrophage, was lost. Time-lapse video, which is inexpensive to run and can be maintained in continuous recording for many weeks, is an ideal technique for recording both erythroid cell pedigrees, and the initial events leading to the formation of an erythroblastic islet in vitro after stimulation with AMS.
    Citation
    Cellular interactions in erythroblastic islands in long-term bone marrow cultures, as studied by time-lapse video. 1991, 17 (1):29-38; discussion 39-43 Blood Cells
    Journal
    Blood Cells
    URI
    http://hdl.handle.net/10541/104680
    PubMed ID
    2018858
    Type
    Article
    Language
    en
    ISSN
    0340-4684
    Collections
    All Paterson Institute for Cancer Research

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