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dc.contributor.authorKan, O
dc.contributor.authorHeyworth, Clare M
dc.contributor.authorDexter, T Michael
dc.contributor.authorMaudsley, P
dc.contributor.authorCook, N
dc.contributor.authorVallance, S
dc.contributor.authorWhetton, Anthony D
dc.date.accessioned2010-06-11T10:37:40Z
dc.date.available2010-06-11T10:37:40Z
dc.date.issued1991-11-15
dc.identifier.citationInterferon-gamma stimulates the survival and influences the development of bipotential granulocyte-macrophage colony-forming cells. 1991, 78 (10):2588-94 Blooden
dc.identifier.issn0006-4971
dc.identifier.pmid1824254
dc.identifier.urihttp://hdl.handle.net/10541/104672
dc.description.abstractThe effects of interferon-gamma (IFN-gamma) on a highly enriched population of granulocyte-macrophage colony-forming cells (GM-CFC) were assessed. When added with myeloid growth factors (interleukin-3 [IL-3], granulocyte-macrophage colony-stimulating factor [GM-CSF], or macrophage-CSF [M-CSF]), IFN-gamma inhibited the formation of colonies in soft agar assays. Furthermore IFN-gamma stimulated an increase in the number of macrophages present in colonies formed in the presence of IL-3. IFN-gamma also inhibited M-CSF-, GM-CSF-, or IL-3-stimulated [3H]-thymidine incorporation in highly enriched GM-CFC. However, when added in the absence of hematopoietic growth factors, IFN-gamma promoted the survival of GM-CFC and had a modest stimulatory effect on DNA synthesis. The direct interaction of the IFN with GM-CFC was confirmed by showing its ability to rapidly activate the sodium/hydrogen antiport in GM-CFC, as do the mitogens GM-CSF, M-CSF, and IL-3. However, the effect of IFN-gamma on intracellular pH and DNA synthesis was transient and pretreatment with IFN markedly inhibited the ability of GM-CSF, M-CSF, and IL-3 to activate the sodium/hydrogen antiport. IFN-gamma has a dual effect on GM-CFC, decreasing the rate of cell death but also limiting the proliferative response to CSFs.
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals
dc.subject.meshBone Marrow Cells
dc.subject.meshCell Survival
dc.subject.meshCells, Cultured
dc.subject.meshColony-Forming Units Assay
dc.subject.meshDNA Replication
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor
dc.subject.meshGranulocytes
dc.subject.meshGrowth Substances
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshHydrogen-Ion Concentration
dc.subject.meshInterferon-gamma, Recombinant
dc.subject.meshInterleukin-3
dc.subject.meshKinetics
dc.subject.meshMacrophage Colony-Stimulating Factor
dc.subject.meshMacrophages
dc.subject.meshMice
dc.subject.meshRecombinant Proteins
dc.subject.meshTime Factors
dc.titleInterferon-gamma stimulates the survival and influences the development of bipotential granulocyte-macrophage colony-forming cells.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biochemistry and Applied Molecular Biology, UMIST, Manchester, UK.en
dc.identifier.journalBlooden
html.description.abstractThe effects of interferon-gamma (IFN-gamma) on a highly enriched population of granulocyte-macrophage colony-forming cells (GM-CFC) were assessed. When added with myeloid growth factors (interleukin-3 [IL-3], granulocyte-macrophage colony-stimulating factor [GM-CSF], or macrophage-CSF [M-CSF]), IFN-gamma inhibited the formation of colonies in soft agar assays. Furthermore IFN-gamma stimulated an increase in the number of macrophages present in colonies formed in the presence of IL-3. IFN-gamma also inhibited M-CSF-, GM-CSF-, or IL-3-stimulated [3H]-thymidine incorporation in highly enriched GM-CFC. However, when added in the absence of hematopoietic growth factors, IFN-gamma promoted the survival of GM-CFC and had a modest stimulatory effect on DNA synthesis. The direct interaction of the IFN with GM-CFC was confirmed by showing its ability to rapidly activate the sodium/hydrogen antiport in GM-CFC, as do the mitogens GM-CSF, M-CSF, and IL-3. However, the effect of IFN-gamma on intracellular pH and DNA synthesis was transient and pretreatment with IFN markedly inhibited the ability of GM-CSF, M-CSF, and IL-3 to activate the sodium/hydrogen antiport. IFN-gamma has a dual effect on GM-CFC, decreasing the rate of cell death but also limiting the proliferative response to CSFs.


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